Barium-induced inhibition of K+ transport mechanisms in cortical astrocytes—its possible contribution to the large Ba2+-evoked extracellular K+ signal in brain
Barium-induced inhibition of K+ transport mechanisms in cortical astrocytes—its possible contribution to the large Ba2+-evoked extracellular K+ signal in brain
复制标题
钡诱导的皮质星形胶质细胞 K+ 转运机制的抑制——其可能对大脑中 Ba2+ 诱发的大细胞外 K+ 信号的贡献
DOI：
10.1016/0306-4522(84)90108-8
复制
发表时间：
1984
期刊：
影响因子：
3.3
通讯作者：
Leif Hertz
中科院分区：
文献类型：
--
作者：
W. Walz;M. Shargool;Leif Hertz
Homogenous mouse astrocytes in primary cultures were used to investigate the action of different Ba2+concentrations on42K transport, membrane potential and Na+,K+-adenosine triphosphatase activity. Five millimolar Ba2+reduced total K+influx and efflux (each by 83%) and ouabain-sensitive net K+uptake (by 80%); it decreased the K+content, depolarized the membrane potential reversibly and completely inhibited the Na+,K+-adenosine triphosphatase activity. The concentration dependence of these effects was biphasic. Concentrations between 2 and 20 μM affected only the passive K+fluxes (IC50:6 μM). Concentrations between 50μM and 5 mM inhibited the Na+,K+-adenosine triphosphatase and had no further effect on passive fluxes, but inhibited the ouabain-sensitive net uptake of K+(IC50: 3.1−0.6mM).It is suggested that the large evoked extracellular K+increase in the brain observed in Ba2+-treated preparationsin vivoor in brain slices to a large extent is due to the impairment of passive and active K+clearance by glial cells.
影响因子：
5.5
作者：
NICHOLSON, C;PHILLIPS, JM
通讯作者：
PHILLIPS, JM