Salivary gland cytokine expression in NOD and normal BALB/c mice
Salivary gland cytokine expression in NOD and normal BALB/c mice
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DOI：
10.1006/clim.1999.4759
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发表时间：
1999-09-01
影响因子：
8.6
通讯作者：
Fox, PC
中科院分区：
文献类型：
--
作者：
Yamano, S;Atkinson, JC;Fox, PC
易患自身免疫性糖尿病的非肥胖性糖尿病(NOD)小鼠发展为慢性淋巴细胞性内分泌腺和外分泌腺浸润。本研究的目的是研究NOD小鼠唾液免疫渗透和细胞因子表达的特点，并与正常BALB/c小鼠进行比较。NOD小鼠的唾液流率在8到12周之间开始下降。同时，在唾液腺中可检测到淋巴细胞灶。NOD小鼠唾液腺淋巴细胞浸润随增龄而增加，同时唾液功能下降。BALB/c小鼠唾液组织中未见淋巴细胞浸润。用逆转录聚合酶链式反应检测NOD和BALB/c小鼠颌下腺中多种炎性细胞因子的信使RNA表达，包括IL-1β、IL-2、IL-10、干扰素-γ和肿瘤坏死因子-cu。IL-4的合成也存在于某些组织中。免疫组织化学分析显示，NOD小鼠淋巴细胞浸润和上皮细胞内均有炎性细胞因子的强烈表达。在平行染色的BALB/c组织中，仅偶尔检测到相同的细胞因子的极少量表达。这些结果证明了细胞因子在正常小鼠唾液腺中的表达，提示这些炎性细胞因子的过度表达可能参与了NOD小鼠唾液腺器官局限性自身免疫的发生和发展。
The autoimmune diabetes-prone nonobese diabetic (NOD) mouse develops a chronic lymphocytic infiltration of endocrine and exocrine glands. The objectives of this study were to characterize the salivary immune infiltration and cytokine expression of NOD mice and compare these findings to those of normal BALB/c mice. A decline in salivary flow rates in NOD mice began between 8 and 12 weeks of age. At this same time lymphocytic foci are detectable in the salivary glands. Lymphocytic infiltration in the salivary glands of NOD mice increased with age and simultaneously salivary function declined. No lymphocytic infiltration was seen in BALB/c salivary tissues. Messenger RNA expression of several inflammatory cytokines, including interleukin-1 beta (IL-1 beta), IL-2, IL-10, interferon-gamma, and tumor necrosis factor-cu was detected in the submandibular glands of both NOD and BALB/c mice by the reverse transcription polymerase chain reaction. IL-4 synthesis was also present in some tissues. Immunohistochemical analysis demonstrated the intense expression of inflammatory cytokines within lymphocytic infiltrates and epithelial cells of all NOD mice. Minimal expression of the same cytokines was detected only occasionally in BALB/c tissues stained in parallel. These results demonstrate cytokine expression in the salivary glands of normal mice and suggest that the overexpression of these inflammatory cytokines is likely involved in the development and progression of the organ-localized autoimmunity in the salivary glands of NOD mice.