A MONOCLONAL-ANTIBODY BLOCKING ELISA TO DETECT SEROTYPE-SPECIFIC INFECTIOUS-BRONCHITIS VIRUS-ANTIBODIES
A MONOCLONAL-ANTIBODY BLOCKING ELISA TO DETECT SEROTYPE-SPECIFIC INFECTIOUS-BRONCHITIS VIRUS-ANTIBODIES
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DOI：
10.1016/0378-1135(93)90015-y
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发表时间：
1993-03-01
影响因子：
3.3
通讯作者：
NAQI, S
中科院分区：
文献类型：
--
作者：
KARACA, K;NAQI, S
建立了单克隆抗体（Mab）阻断酶联免疫吸附试验（B-ELISA）检测鸡传染性支气管炎病毒（IBV）血清型特异性抗体的方法，并与传统的间接ELISA（I-ELISA）和病毒中和试验（VN）进行了比较。本研究中使用的血清来源于实验性接种三种最流行的IBV血清型的鸡，即阿肯色州（方舟）、康涅狄格州（康涅狄格州）和马萨诸塞州（马萨诸塞州）。在免疫反应的初级和次级阶段，两者都检测到鸡血清中的血型特异性抗体。两种试验的结果均表明，在对IBV血清型的初次应答期间产生的抗体具有很强的亚型特异性。在二次应答期间产生的那些与同源病毒反应更强烈，但确实表现出与异源抗原的一定水平的交叉反应性。I-ELISA检测IBV群特异性抗体而非群特异性抗体，B-ELISA既具有I-ELISA的方便性，又具有VN试验的血清型特异性，在IBV的现场诊断和IBV疫苗免疫效果评价中具有良好的应用前景。
A monoclonal antibody (Mab) blocking enzyme-linked immunosorbent assay (B-ELISA) was developed and compared to a conventional indirect ELISA (I-ELISA) and a virus-neutralization (VN) test for detection of specific antibodies to avian infectious bronchitis virus (IBV) serotypes. Sera used in this study were derived from chickens experimentally inoculated with the three most prevalent IBV serotypes, Arkansas (Ark), Connecticut (Conn), and Massachusetts (Mass).Overall, there was good correlation between the results of B-ELISA and the VN test. Both detected serotype-specific antibodies in chicken sera during primary and secondary phases of the immune response. Results of both tests indicated that the antibodies produced during the primary response to IBV serotypes are strongly serotype-specific. Those produced during the secondary response react more strongly with the homologous virus, but do exhibit some level of cross-reactivity with heterologous antigens. I-ELISA detected IBV group-specific and not serotype-specific antibodies.The B-ELISA which both offers the convenience of the conventional I-ELISA and the serotype specificity of the VN test, holds excellent promise for field application in IBV diagnosis and evaluation of response to IBV vaccines.