Expression of heat shock proteins (HSP27, HSP60, HSP70, HSP90, GRP78, GRP94) in hepatitis B virus-related hepatocellular carcinomas and dysplastic nodules
Expression of heat shock proteins (HSP27, HSP60, HSP70, HSP90, GRP78, GRP94) in hepatitis B virus-related hepatocellular carcinomas and dysplastic nodules
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DOI：
10.3748/wjg.v11.i14.2072
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发表时间：
2005-04-14
影响因子：
4.3
通讯作者：
Park, Cheol Keun
中科院分区：
文献类型：
--
作者：
Lim, Seung Oe;Park, Sung Gyoo;Park, Cheol Keun
目的：热休克蛋白（HSPs）在肝细胞癌（HCC）中的表达频繁上调，HCC从不典型增生结节（DN）和早期HCC发展到晚期HCC。然而，对HSPs在多步骤肝癌发生中的差异表达知之甚少。这是本研究的目的是监测热休克蛋白的表达在多步骤肝癌发生和评估其预后的意义，在肝炎B病毒（HBV）相关hcch.METHODS：38 HCC和19 DN样本从52例B表面抗原阳性的韩国患者。结果：HSP27、HSP60、HSP70、HSP90、GRP 78、GRP 94的表达随肝癌发生发展的进程而沿着增加，HSP27、HSP70、HSP90、HSP90、GRP 78、GRP 94的表达随肝癌发生发展的进程而增加。仅在GRP78中发现强相关性（斯皮尔曼r = 0.802）。GRP78、GRP94、HSP90、HSP70的表达与HCC的预后因素呈正相关。结论：HSPs的表达在HBV相关肝癌中普遍上调，GRP 78、GRP 94或HSP 90的表达可能在HBV相关肝癌的逐步进展中发挥重要作用。GRP78、GRP94和HSP 90可能是HBV相关HCC的重要预后标志物，强烈提示血管浸润和肝内转移。(C)2005年WJG出版社和Elsevier Inc. All rights reserved.
AIM: Expression of heat shock proteins (HSPs) is frequently up-regulated in hepatocellular carcinoma (HCC), which evolves from dysplastic nodule (DN) and early HCC to advanced HCC. However, little is known about the differential expression of HSPs in multistep hepatocarcinogenesis. It was the purpose of this study to monitor the expression of HSPs in multistep hepatocarcinogenesis and to evaluate their prognostic significance in hepatitis B virus (HBV)-related HCC.METHODS: Thirty-eight HCC and 19 DN samples were obtained from 52 hepatitis B surface antigen-positive Korean patients. Immunohistochemical and dot immunoblot analyses of HSP27, HSP60, HSP70, HSP90, glucose regulated protein (GRP)78, and GRP94 were performed and their expression at different stages of HCC development was statistically analyzed.RESULTS: Expression of HSP27, HSP70, HSP90, GRP78, and GRP94 increased along with the stepwise progression of hepatocarcinogenesis. Strong correlation was found only in GRP78 (Spearman's r = 0.802). There was a positive correlation between the expressions of GRP78, GRP94, HSP90, or HSP70 and prognostic factors of HCC. Specifically, the expression of GRP78, GRP94, or HSP90 was associated significantly with vascular invasion and intrahepatic metastasis.CONCLUSION: The expressions of HSPs are commonly up-regulated in HBV-related HCCs and GRP78 might play an important role in the stepwise progression of HBV-related hepatocarcinogenesis. GRP78, GRP94, and HSP90 may be important prognostic markers of HBV-related HCC, strongly suggesting vascular invasion and intrahepatic metastasis. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved.