IMMUNOHISTOCHEMICAL DETECTION OF LAMININ IN 98 HUMAN-BREAST CARCINOMAS - A LIGHT AND ELECTRON-MICROSCOPIC STUDY
IMMUNOHISTOCHEMICAL DETECTION OF LAMININ IN 98 HUMAN-BREAST CARCINOMAS - A LIGHT AND ELECTRON-MICROSCOPIC STUDY
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DOI：
10.1016/s0046-8177(86)80458-0
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发表时间：
1986-04-01
期刊：
影响因子：
3.3
通讯作者：
TOGA, M
中科院分区：
文献类型：
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作者：
CHARPIN, C;LISSITZKY, JC;TOGA, M
采用抗层粘连蛋白和抗生物素蛋白-生物素-过氧化物酶复合物法研究了98例乳腺癌中层粘连蛋白的分布。在血管和上皮基底膜内观察到层粘连蛋白。层粘连蛋白在导管内癌中呈连续线状分布，在浸润性癌中呈不连续线状分布。未检测到细胞内层粘连蛋白染色。电镜下可见非肿瘤性乳腺组织基底膜致密层内有层粘连蛋白免疫染色。在肿瘤中，层粘连蛋白免疫染色经常显示多层基底膜和异常的多层基底膜的血管中的肿瘤间质。这些数据表明，层粘连蛋白免疫染色，作为一种新的方法发生在癌的异质性基底膜的变化，应允许更好地了解细胞扩散过程和间质-肿瘤细胞的相互作用。层粘连蛋白在细胞外与气孔接触的一致分布表明气孔在基底膜组件的组装中起重要作用。
The distribution of laminin was studied in 98 breast carcinomas with antilaminin and the avidin-biotin-peroxidase complex method. Laminin was observed within vascular and epithelial basement membranes. Laminin displayed a continuous linear pattern in intraductal carcinomas, and it was heterogeneously distributed, with a discontinuous linear pattern, in invasive carcinomas. No intracellular laminin staining was detected. Electron microscopic study showed laminin immunostaining in the lamina densa of basement membranes in nonneoplastic breast tissue. In tumors, laminin immunostaining frequently revealed multilayered basement membranes and abnormal multilayered basement membranes in blood vessels in the tumor stroma. These data suggest that laminin immunostaining, as a new approach to the heterogeneous basement membrane changes occurring in carcinomas, should permit better understanding of cell diffusion processes and of stroma-tumor cell interactions. The consistent extracellular distribution of laminin in contact with the stoma indicates that the latter plays and important role in the assembly of basement membrane components.