Adventitial changes in pulmonary vasculature in congenital diaphragmatic hernia complicated by pulmonary hypertension
Adventitial changes in pulmonary vasculature in congenital diaphragmatic hernia complicated by pulmonary hypertension
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DOI：
10.1016/s0022-3468(98)90468-7
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发表时间：
1998-02-01
影响因子：
2.4
通讯作者：
Puri, P
中科院分区：
文献类型：
--
作者：
Taira, Y;Yamataka, T;Puri, P
目的：本研究的目的是描述先天性腹股沟疝（CDH）合并持续性肺动脉高压（PPH）患者肺血管结构的变化，特别强调外膜厚度。方法：维多利亚蓝货车Gieson（WG）染色和抗α平滑肌肌动蛋白（ASMA）免疫染色对23例CDH合并PPH患者和11例年龄匹配的婴儿猝死综合征（SIDS）患者的尸检肺组织进行了检测。用IPS-4.01型图像分析仪测量肺动脉外径（艾德）小于75 μ m、75 ~ 100 μ m、100 ~ 150 μ m、150 ~ 250 μ m、250 ~ 500 μ m和大于500 μ m的中、外膜增厚程度，并进行统计学比较。在艾德径小于100 μ m、100 - 200 μ m和大于200 μ m的肺静脉中也测量了中膜增厚和外膜增厚的程度。为了确定是否特征性的结构变化的大小相关，每一个都与ED。外膜和介质的肺动脉和静脉的面积进行了测量，使用图像analyzer.Results：有一个显着增加中膜和外膜厚度在所有大小的动脉CDH患者与对照组相比（P <0.01）。与对照组相比，CDH患者所有尺寸的动脉的外膜面积程度显著增加（P <0.01），而仅小于100 μ m尺寸的动脉的中膜面积程度显著增加（P <0.05）。计算每条动脉壁中各种成分的面积表明，对于小动脉（
Purpose: The purpose of this study was to characterize structural changes in the pulmonary vasculature in congenital diaphragmatic hernia (CDH) complicated by persistent pulmonary hypertension (PPH) with particular emphasis on adventitial thickness.Methods: Victorian blue Van Gieson (WG) staining and immunostaining with antialpha smooth muscle actin (ASMA) were performed on lung tissues obtained at autopsy from 23 patients with CDH complicated by PPH and 11 age-matched control tissues of sudden infant death syndrome patients (SIDS). The degree of medial and adventitial thickening was measured in pulmonary arteries with an external diameter (ED) of less than 75 mu m, 75 to 100 mu m, 100 to 150 mu m, 150 to 250 mu m, 250 to 500 mu m, and greater than 500 mu m by IPS-4.01 image analyzer and compared statistically. The degree of medial thickening and adventitial thickening was also measured in pulmonary veins with an ED of less than 100 mu m, 100 to 200 mu m, and greater than 200 mu m. To determine whether the characteristic structural changes were size related, each was related to ED. The area of adventitia and media of the pulmonary arteries and veins was measured using image analyzer.Results: There was a significant increase in medial and adventitial thickness in arteries of all sizes in CDH patients compared with controls (P < .01). The degree of adventitial area was significantly increased for arteries of all sizes (P < .01) and the degree of medial area was significantly increased only for arteries less than 100 pm size (P < .05) in CDH patients compared with controls. Calculation of the areas of the various components in the wall of each artery showed that for small arteries (