Genetic effects of radio-frequency, atmospheric-pressure glow discharges with helium
Genetic effects of radio-frequency, atmospheric-pressure glow discharges with helium
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DOI：
10.1063/1.2938692
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发表时间：
2008-06-02
影响因子：
4
通讯作者：
Bao, Cheng-Yu
中科院分区：
文献类型：
--
作者：
Li, Guo;Li, He-Ping;Bao, Cheng-Yu
大气压辉光放电（APGD）由于气体温度低、活性物质密度高，在等离子体灭菌、基因突变等领域具有潜在的应用前景。本文介绍了以质粒DNA和寡核苷酸为处理对象的氦射频APGD等离子体的遗传效应。实验结果表明，是化学活性物质，而不是热，紫外线辐射，强电场，和/或带电粒子，打破了质粒DNA的双链。遗传效应取决于等离子体操作参数，例如，功率输入、氦气流量、加工距离、时间等。（C）2008美国物理学会。
Due to low gas temperatures and high densities of active species, atmospheric-pressure glow discharges (APGDs) would have potential applications in the fields of plasma-based sterilization, gene mutation, etc. In this letter, the genetic effects of helium radio-frequency APGD plasmas with the plasmid DNA and oligonucleotide as the treated biomaterials are presented. The experimental results show that it is the chemically active species, instead of heat, ultraviolet radiation, intense electric field, and/or charged particles, that break the double chains of the plasmid DNA. The genetic effects depend on the plasma operating parameters, e.g., power input, helium flow rate, processing distance, time, etc. (C) 2008 American Institute of Physics.