Altered formation of the iron oxide nanoparticle-biocorona due to individual variability and exercise.
Altered formation of the iron oxide nanoparticle-biocorona due to individual variability and exercise.
复制标题
由于个体差异和运动，氧化铁纳米颗粒生物冠的形成发生了改变。
DOI：
10.1016/j.etap.2018.07.014
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发表时间：
2018
影响因子：
4.3
通讯作者：
Shannahan,JonathanH
中科院分区：
文献类型：
--
作者：
Kobos,LisaM;Adamson,SherleenXue-Fu;Evans,Sheelagh;Gavin,TimothyP;Shannahan,JonathanH
纳米颗粒（NP），引入到生物环境中，积累生物分子或生物冠（BC）的涂层。虽然BC具有毒理学和药理学后果，但个体间变异性和运动对NP-BC形成的影响尚不清楚。我们假设在血浆中孵育的NP在个体之间形成不同的BC，并且运动引起额外的个体内改变。将20 nm氧化铁（Fe 3 O 4）纳米颗粒在运动前或运动后的血浆中离体孵育，并利用蛋白质组学来评估BC组分。分析表明，不同的个人之间的BC形成，而运动被发现，以提高NP-BC的复杂性。测定了不同个体间及运动后NP-BC蛋白的显著差异。由于NP-BC的变异性，鉴别出不同的人巨噬细胞应答。这些发现表明，个体形成独特的BC，运动影响NP-生物分子相互作用。NP-生物分子相互作用的理解是必要的，负责在NP暴露和/或基于纳米的疗法的人类反应的变化的机制的阐明。
Nanoparticles (NPs), introduced into a biological environment, accumulate a coating of biomolecules or biocorona (BC). Although the BC has toxicological and pharmacological consequences, the effects of inter-individual variability and exercise on NP-BC formation are unknown. We hypothesized that NPs incubated in plasma form distinct BCs between individuals, and exercise causes additional intra-individual alterations. 20 nm iron oxide (Fe3O4) NPs were incubated in pre- or post-exercise plasmaex vivo, and proteomics was utilized to evaluate BC components. Analysis demonstrated distinct BC formation between individuals, while exercise was found to enhance NP-BC complexity. Abundance differences of NP-BC proteins were determined between individuals and resulting from exercise. Differential human macrophage response was identified due to NP-BC variability. These findings demonstrate that individuals form unique BCs and that exercise influences NP-biomolecule interactions. An understanding of NP-biomolecule interactions is necessary for elucidation of mechanisms responsible for variations in human responses to NP exposures and/or nano-based therapies.