Expression of Novel CD44st and MMP2 in NSCLC Tissues and Their Clinical Significance
Expression of Novel CD44st and MMP2 in NSCLC Tissues and Their Clinical Significance
复制标题
DOI：
10.1159/000456669
复制
发表时间：
2017-04-01
影响因子：
2.4
通讯作者：
Fang, Xin Jian
中科院分区：
文献类型：
--
作者：
Zhang, Lei;Li, Ning;Fang, Xin Jian
背景：CD44分子在肿瘤组织中的异常表达可导致细胞外基质的降解，并与腋窝淋巴结转移、血管生成、肿瘤进展及耐药密切相关。方法：应用定量逆转录聚合酶链式反应、基因测序和免疫组织化学方法，检测72例腺癌和53例鳞癌患者术后非小细胞肺癌(NSCLC)癌组织和癌旁正常组织中CD44st和MMP2mRNAs和蛋白的表达。结果：CD44st和MMP2的表达与肿瘤的组织学类型、淋巴结转移和TNM分期密切相关，差异有统计学意义(P<；0.05)。CD44st高表达组的中位总生存期为30.52月(95%可信区间为24.02-36.15)，低表达组为43.23个月(95%可信区间为31.81-52.02)(p=0.020)。MMP2高表达组的中位OS为30.53个月(95%CLA26.69~33.31)，低表达组为40.06个月(95%CLA33.55~46.45)(P=0.022)。结论：CD44st和MMP2在鳞癌中的表达明显高于腺癌，与淋巴结转移和TNM分期密切相关，并影响患者的预后。(C)2017年，弗莱堡S.Karger GmbH
Background: Abnormal expression of some CD44 molecules in tumor tissues can induce the degradation of the extracellular matrix, and is closely associated with axillary lymph node metastasis, angiogenesis, cancer progression, and drug resistance. Patients and Methods: We measured and confirmed the expression of CD44st and MMP2 mRNAs and proteins in the cancer tissues and adjacent normal tissues of postoperative non-small cell lung cancer (NSCLC) patients with either adenocarcinoma (n = 72) or squamous cell carcinoma (n = 53) using quantitative reverse transcription polymerase chain reaction, gene sequencing, and immunohistochemical analysis. Results: CD44st and MMP2 expression were closely associated with the histopathological classification, lymph node metastasis, and TNM stage of the tumors, and the difference was statistically significant (p < 0.05). The median overall survival (OS) for the high CD44st expression group was 30.52 months (95% confidence interval (Cl) 24.02-36.15); for the low expression group it was 43.23 months (95% Cl 31.81-52.02) (p = 0.020). The median OS for the high MMP-2 expression group was 30.53 months (95% Cl 26.69-33.31); for the low expression group it was 40.06 months (95% Cl 33.55-46.45) (p = 0.022). Conclusion: The rates of CD44st and MMP2 expression were higher in squamous cell carcinomas than in adenocarcinomas, were closely associated with lymph node metastasis and TNM stage, and affected patients' prognoses. (C) 2017 S. Karger GmbH, Freiburg