Co-Circulation of Canine Coronavirus I and IIa/b with High Prevalence and Genetic Diversity in Heilongjiang Province, Northeast China.
Co-Circulation of Canine Coronavirus I and IIa/b with High Prevalence and Genetic Diversity in Heilongjiang Province, Northeast China.
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中国东北黑龙江省犬冠状病毒 I 型和 IIa/b 型的高流行性和遗传多样性的共同传播
DOI：
10.1371/journal.pone.0146975
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Sun D
中科院分区：
文献类型：
--
作者：
Wang X;Li C;Guo D;Wang X;Wei S;Geng Y;Wang E;Wang Z;Zhao X;Su M;Liu Q;Zhang S;Feng L;Sun D
为追踪犬冠状病毒（CCoV）的进化，对201份东北地区健康犬粪便标本进行了针对CCoV部分M和S基因的逆转录-聚合酶链反应（RT-PCR），并进行了流行病学分析。M基因RT-PCR检测结果显示，28.36%（57/201）的样本为CCoV阳性，其中CCoV-I型和CCoV-II型分别占15.79%（9/57）和84.21%（48/57）。序列分析表明，57株CCoV的M基因部分核苷酸同源性为88.4%~ 100%，与中国参考株HF 3的同源性为88.7%~ 96.2%。CCoV-I和CCoV-II株与中国和其他国家的参考株相比具有遗传多样性。57株CCoV与犬小病毒2型（CPV-2）和犬库布病毒（CaKV）的混合感染率分别为31.58%和33.33%。不同免疫状态、地区、季节和年龄的犬CCoV感染率存在显著差异。从57份CCoV阳性标本中扩增出28条S基因，其中26株CCoV-IIa，1株CCoV-IIb，1株CCoV-I。序列分析表明，26株CCoV-IIa株S基因部分序列的同源性为86.3%~ 100%，与中国参考株V1的同源性为89.6%~ 92.2%。26株CCoV-IIa株与国内外参考株比较，显示出遗传多样性。结果表明，我国东北地区犬群中存在CCoV-I、CCoV-IIa、CCoV-IIb共感染，且CCoV-II与CaKV、CPV-2共感染率较高，CCoV-II具有较高的流行率和遗传多样性。
To trace the evolution of canine coronavirus (CCoV), 201 stool samples from diarrheic dogs in northeast China were subjected to reverse transcription-polymerase chain reactions (RT-PCRs) targeting the partial M and S genes of CCoV, followed by an epidemiological analysis. M gene RT-PCRs showed that 28.36% (57/201) of the samples were positive for CCoV; of the 57 positive samples, CCoV-I and CCoV-II accounted for 15.79% (9/57) and 84.21% (48/57), respectively. A sequence comparison of the partial M gene revealed nucleotide homologies of 88.4%–100% among the 57 CCoV strains, and 88.7%–96.2% identity between the 57 CCoV strains and the Chinese reference strain HF3. The CCoV-I and CCoV-II strains exhibited genetic diversity when compared with reference strains from China and other countries. The 57 CCoV strains exhibited high co-infection rates with canine kobuvirus (CaKV) (33.33%) and canine parvovirus-2 (CPV-2) (31.58%). The CCoV prevalence in diarrheic dogs differed significantly with immunization status, regions, seasons, and ages. Moreover, 28 S genes were amplified from the 57 CCoV-positive samples, including 26 CCoV-IIa strains, one CCoV-IIb strain, and one CCoV-I strain. A sequence comparison of the partial S gene revealed 86.3%–100% nucleotide identity among the 26 CCoV-IIa strains, and 89.6%–92.2% identity between the 26 CCoV-IIa strains and the Chinese reference strain V1. The 26 CCoV-IIa strains showed genetic diversity when compared with reference strains from China and other countries. Our data provide evidence that CCoV-I, CCoV-IIa, and CCoV-IIb strains co-circulate in the diarrhoetic dogs in northeast China, high co-infection rates with CaKV and CPV-2 were observed, and the CCoV-II strains exhibited high prevalence and genetic diversity.