Simple method for the culture of glial cells from embryonic rat brain: implications for regional heterogeneity and the radial glial lineage.
Simple method for the culture of glial cells from embryonic rat brain: implications for regional heterogeneity and the radial glial lineage.
复制标题
胚胎大鼠脑神经胶质细胞培养的简单方法：对区域异质性和放射状神经胶质谱系的影响。
DOI：
10.1002/jnr.490210214
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发表时间：
1988
影响因子：
4.2
通讯作者：
Lauder,JM
中科院分区：
文献类型：
--
作者：
Wilkie,MB;Lauder,JM
描述了一种简单的方法，用于从第13-15天的胚胎大鼠脑干和中脑的特定区域生产神经胶质细胞培养物，其基于对相对非粘附性基质的差异细胞附着，其抑制神经元的生长。脑干和中脑细胞的特定群体在基底上附着和扩散的能力的区域差异表明，胚胎神经胶质细胞群体的细胞表面性质可能不同，即使它们来自发育中大脑的相同区域。基于对放射状胶质细胞和星形胶质细胞在体外随时间的时空分布的观察，我们认为这种培养系统可能被证明对放射状胶质细胞谱系的研究有用。
A simple method is described for the production of glial cell cultures from specific regions of the day 13–15 embryonic rat brainstem and midbrain based on differential cell attachment to a relatively nonadhesive substrate, which inhibits the growth of neurons. Regional differences in the ability of specific populations of brainstem and midbrain cells to attach and spread on the substrate suggest that embryonic glial populations may differ in their cell surface properties even when they derive from the same general area of the developing brain. Based on observations of the spatiotemporal distribution of radial‐like glial cells and astrocytes with time in vitro, we suggest that this culture system may prove useful for investigation of the radial glial lineage.