A novel method for growing Arabidopsis thaliana plants hydroponically
A novel method for growing Arabidopsis thaliana plants hydroponically
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DOI：
10.1034/j.1399-3054.2000.108002188.x
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发表时间：
2000-02-01
影响因子：
6.4
通讯作者：
Arteca, JM
中科院分区：
文献类型：
--
作者：
Arteca, RN;Arteca, JM
在这份报告中，我们描述了一个简单的系统的发展，在环境条件下，这是简单的维护，同时最大限度地提高生长和发展的个人水培拟南芥植物。该系统包括一个救生筏漂浮装置，其中心插入一个14-20毫米的海绵;将该单元置于含有拟南芥营养液的洋红GA 7容器中，在该实验系统中生长的植物不需要通气，125 μ mol m（-2）s（-1）支持植物旺盛生长，1/4至18浓度的拟南芥营养液促进最大生长，而更高或更低的水平具有不利影响，并且发现18至24摄氏度之间的温度是最佳的。在这些条件下生长的植物表现出与在盆栽培养基中生长的植物相似的生理因素，例如开花时间和整体外观。本文所述的低维护方法提供了一种替代现有的水培方法用于生长拟南芥。本文还讨论了该系统在一个容器中种植大量植物的适应性。
In this report we describe the development of a simple system for growing individual Arabidopsis plants hydroponically under environmental conditions which are simple to maintain while maximizing growth and development. The system consists of a Life Raft Float Unit with a 14-20 mm sponge inserted into its center; this unit is placed into a Magenta GA7 vessel containing Arabidopsis nutrient solution, Plants grown in this experimental system do not require aeration, 125 mu mol m(-2) s(-1) supports vigorous plant growth, 1/4 to 1/8 strength Arabidopsis nutrient solution promotes maximal growth while higher or lower levels have adverse effects and temperatures between 18 and 24 degrees C were found to be optimal. Plants grown under these conditions exhibit physiological factors such as time to flowering and overall appearance similar to those grown in potting media. The low maintenance method described in this paper provides an alternative to existing hydroponic methods used to grow Arabidopsis. Adaptation of this system for growing large numbers of plants in 1 container is also discussed.