Functional diversification upon leader protease domain duplication in the Citrus tristeza virus genome: Role of RNA sequences and the encoded proteins
Functional diversification upon leader protease domain duplication in the Citrus tristeza virus genome: Role of RNA sequences and the encoded proteins
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DOI：
10.1016/j.virol.2017.11.014
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发表时间：
2018-01-15
期刊：
影响因子：
3.7
通讯作者：
Folimonova, Svetlana Y.
中科院分区：
文献类型：
--
作者：
Kang, Sung-Hwan;Atallah, Osama O.;Folimonova, Svetlana Y.
来自梭状病毒科的病毒显示了一个以上基因的基因组内复制的例子。除了标志性的外壳蛋白基因重复外，一些成员还具有木瓜蛋白酶样前导蛋白酶的串联重复。在这项研究中，我们证明了结构域编码的L1和L2蛋白酶在柑橘衰退病毒基因组中经历了显着的功能分歧，在RNA和蛋白质水平。我们表明，L1蛋白酶是至关重要的病毒积累和建立初始感染，而其编码区是至关重要的病毒运输。另一方面，第二蛋白酶是不可或缺的病毒感染的天然柑橘类宿主，这表明L2已经进化出一个重要的适应功能，介导病毒与木本宿主的相互作用。
Viruses from the family Closteroviridae show an example of intra-genome duplications of more than one gene. In addition to the hallmark coat protein gene duplication, several members possess a tandem duplication of papainlike leader proteases. In this study, we demonstrate that domains encoding the L1 and L2 proteases in the Citrus tristeza virus genome underwent a significant functional divergence at the RNA and protein levels. We show that the L1 protease is crucial for viral accumulation and establishment of initial infection, whereas its coding region is vital for virus transport. On the other hand, the second protease is indispensable for virus infection of its natural citrus host, suggesting that L2 has evolved an important adaptive function that mediates virus interaction with the woody host.