Early neurogenesis during caudal spinal cord regeneration in adult Gekko japonicus
Early neurogenesis during caudal spinal cord regeneration in adult Gekko japonicus
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DOI：
10.1007/s10735-012-9466-3
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发表时间：
2012-11
影响因子：
3.2
通讯作者：
Youlang Zhou;Qingsong Xu;Donghui Li;Lijuan Zhao;Yongjun Wang;Mei Liu;X. Gu;Yan Liu
中科院分区：
文献类型：
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作者：
Youlang Zhou;Qingsong Xu;Donghui Li;Lijuan Zhao;Yongjun Wang;Mei Liu;X. Gu;Yan Liu
日本壁虎尾部脊髓在截尾后发生了戏剧性的变化。截肢可诱导尾侧室管膜管的细胞增殖。在再生过程中的不同时间点进行苏木精-伊红染色，观察再生附件的形态特征。截肢后中央管延伸至胚泡，伤后3周出现软骨和肌肉组织。用溴脱氧尿嘧啶核苷(BrdU)掺入法检测再生过程中的增殖细胞。在中央管周可见BrdU阳性细胞。应用巢蛋白和神经元特异性烯醇化酶(NSE)免疫细胞化学方法检测神经干/祖细胞和神经元。损伤后2周，Nestin阳性细胞位于室管膜管外，截肢后3周出现NSE阳性细胞。这些数据表明，神经发生是壁虎尾侧脊髓再生的早期事件。
Gekko japonicus undergoes dramatic changes in the caudal spinal cord after tail amputation. The amputation induces cell proliferation in the caudal ependymal tube. We performed hematoxylin and eosin staining at different time points in the regeneration process to investigate the morphological characterization of the regenerated appendages. The central canal extended to the blastema post-amputation and the cartilage and muscle tissue appeared 3 weeks after injury. We performed the bromodeoxyuridine (BrdU) incorporation assay to detect proliferating cells during the regeneration process. BrdU positive cells were detected in the peri-central canal. Furthermore, nestin and neuron-specific enolase (NSE) immunocytochemistry were applied to detect neural stem/progenitor cells and neurons. Two weeks after injury, nestin-positive cells undergoing proliferation were located outside of the ependymal tube, and NSE positive cells appeared after 3 weeks of amputation. These data suggest that neurogenesis is an early event during caudal spinal cord regeneration in gecko.