The distribution of active force generators controls mitotic spindle position
The distribution of active force generators controls mitotic spindle position
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DOI：
10.1126/science.1086560
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发表时间：
2003-07-25
期刊：
影响因子：
56.9
通讯作者：
Hyman, A
中科院分区：
文献类型：
--
作者：
Grill, SW;Howard, J;Hyman, A
在不均等的细胞分裂过程中，有丝分裂纺锤体在细胞分裂之前偏心定位。为了确定导致单细胞秀丽隐杆线虫胚胎纺锤体移位的净力不平衡的基础，我们用紫外激光将中心体片段化。碎片速度的平均值和方差的分析表明，力的不平衡是由于大量的力发生器上的后星状体相对于前星状体的星形微管拉。此外，异源三聚体鸟嘌呤核苷酸结合蛋白（G蛋白）α亚基的激活是产生这些星体力所必需的。
During unequal cell divisions a mitotic spindle is eccentrically positioned before cell cleavage. To determine the basis of the net force imbalance that causes spindle displacement in one-cell Caenorhabditis elegans embryos, we fragmented centrosomes with an ultraviolet laser. Analysis of the mean and variance of fragment speeds suggests that the force imbalance is due to a larger number of force generators pulling on astral microtubules of the posterior aster relative to the anterior aster. Moreover, activation of heterotrimeric guanine nucleotide-binding protein (G protein) alpha subunits is required to generate these astral forces.