Photoluminescent graphene quantum dots for in vitro and in vivo bioimaging using long wavelength emission
Photoluminescent graphene quantum dots for in vitro and in vivo bioimaging using long wavelength emission
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使用长波长发射进行体外和体内生物成像的光致发光石墨烯量子点
DOI：
10.1039/c5ra02961a
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发表时间：
2015
期刊：
影响因子：
3.9
通讯作者：
Yang Bai
中科院分区：
文献类型：
--
作者：
Zhu Shoujun;Zhou Nan;Hao Zeyu;Maharjan Suraj;Zhao Xiaohuan;Song Yubin;Sun Bin;Zhang Kai;Zhang Junhu;Sun Hongchen;Lu Laijin;Yang Bai
石墨烯量子点（GQDs）具有超小的尺寸、优异的光学性能、化学稳定性、生物相容性、抗光漂白性以及低毒性等优点，被广泛用作荧光生物探针。在本研究中，我们使用自上而下的“纳米切割”路线来制备荧光GQD。所制备的GQD具有ca. 4nm直径，晶格常数为0.218nm，并且由于其上存在许多基于氧和氮的基团而具有突出的溶解性。GQDs被用于体外和体内生物成像。以大鼠雪旺细胞为模型系统，采用荧光激活细胞分选仪（FACS）和激光共聚焦扫描显微镜（CLSM）检测GQDs的时间依赖性细胞摄取，24 h后达到饱和点。利用GQDs的荧光激发依赖性，实现了多色细胞标记，证明GQDs主要分布在细胞质和溶酶体中。此外，使用长波长发射（620 nm），在裸鼠中实现体内成像。
Graphene quantum dots (GQDs), due to their ultrasmall size, excellent optical properties, chemical stability, biocompatibility, anti-photobleaching as well as low toxicity, have been widely used as fluorescent bio-probes. In this study, we used the top-down “nano-cutting” route to prepare fluorescent GQDs. The as-prepared GQDs possessed ca. 4 nm diameter with 0.218 nm crystal lattice constant, and they had outstanding solubility as many oxygen and nitrogen based groups were present on them. The GQDs were exploited in bioimaging in vitro and in vivo. Using rat Schwann cells as the model system, the time-dependent cellular uptake of the GQDs was tested by a fluorescence activated cell sorter (FACS) and confocal laser scanning microscope (CLSM), and it reached the saturation point after 24 h. Benefiting from the excitation-dependent PL of the GQDs, multi-color cell labeling was achieved, and the GQDs were proved to be mainly distributed in the cytoplasm and lysosomes. Furthermore, using long wavelength emission (620 nm), in vivo imaging was realized in nude mice.