Dietary zinc supplementation attenuates hyperglycemia in db/db mice
Dietary zinc supplementation attenuates hyperglycemia in db/db mice
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DOI：
10.1177/153537020122600107
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发表时间：
2001-01-01
影响因子：
3.2
通讯作者：
Taylor, CG
中科院分区：
文献类型：
--
作者：
Simon, SF;Taylor, CG
虽然锌（Zn）缺乏与胰岛素抵抗有关，并且在糖尿病中可能存在锌代谢改变（例如，高锌血症，低正常血浆锌浓度），但锌在II型糖尿病中调节胰岛素作用的潜在作用尚未确定。本研究的目的是比较膳食锌缺乏和锌补充对db/db小鼠血糖控制的影响。断奶db/db小鼠和瘦仔鼠对照组分别饲喂缺锌（3 ppm Zn； dbZD和InZD组）、足锌对照组（30 ppm Zn； dbC和inc组）或补锌（300 ppm Zn； dbZS和InZS组）饲粮6周。测定小鼠锌含量、糖尿病血清和尿指标、腓肠肌胰岛素受体浓度和酪氨酸激酶活性。与dbZD组相比，dbZS组的空腹血糖浓度显著降低（分别为19.3 +/- 2.9 mM和27.9 +/- 4.1 mM），而dbC组则处于中间值。股骨锌与血清葡萄糖浓度呈负相关（r = -0.59, P = 0.007）， dbZS组胰腺锌含量高于dbZC组，循环胰岛素浓度低于dbZC组。在db/db基因型中，胰岛素刺激的腓肠肌酪氨酸激酶活性较高，而胰岛素受体浓度没有改变。综上所述，饲粮中添加锌可减轻db/db小鼠的高血糖和高胰岛素血症，提示锌在胰腺功能和外周组织葡萄糖摄取中的作用有待进一步研究。
Although zinc (Zn) deficiency has been associated with insulin resistance, and altered Zn metabolism (e.g., hyperzincuria, low-normal plasma Zn concentrations) may be present in diabetes, the potential effects of Zn on modulation of insulin action in Type II diabetes have not been established. The objective of this study was to compare the effects of dietary Zn deficiency and Zn supplementation on glycemic control in db/db mice. Weanling db/db mice and lean littermate controls were fed Zn-deficient (3 ppm Zn; dbZD and InZD groups), Zn-adequate control (30 ppm Zn; dbC and inc groups) or Zn-supplemented (300 ppm Zn; dbZS and InZS groups) diets for 6 weeks. Mice were assessed for Zn status, serum and urinary indices of diabetes, and gastrocnemius insulin receptor concentration and tyrosine kinase activity. Fasting serum glucose concentrations were significantly lower in the dbZS group compared with the dbZD group (19.3 +/- 2.9 and 27.9 +/- 4.1 mM, respectively), whereas the dbC mice had an intermediate value. There was a negative correlation between femur Zn and serum glucose concentrations (r = -0.59 for lean mice, P = 0.007), The dbZS group had higher pancreatic Zn and lower circulating insulin concentrations than dbZC mice. Insulin-stimulated tyrosine kinase activity in gastrocnemius muscle was higher in the db/db genotype, and insulin receptor concentration was not altered. In summary, dietary Zn supplementation attenuated hyperglycemia and hyperinsulinemia in db/db mice, suggesting that the roles of Zn in pancreatic function and peripheral tissue glucose uptake need to be further investigated.