Temporal Study of the Perturbation of Crustacean Neuropeptides Due to Severe Hypoxia Using 4-Plex Reductive Dimethylation
Temporal Study of the Perturbation of Crustacean Neuropeptides Due to Severe Hypoxia Using 4-Plex Reductive Dimethylation
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DOI：
10.1021/acs.jproteome.9b00787
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发表时间：
2020-04-03
影响因子：
4.4
通讯作者：
Li, Lingjun
中科院分区：
文献类型：
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作者：
Buchberger, Amanda R.;Sauer, Christopher S.;Li, Lingjun
缺氧（即低氧 (O-2) 水平）是多种水生物种（包括鱼类和无脊椎动物）面临的常见环境挑战。为了生存或逃避这些条件，这些动物已经发展出新的生物机制，其中一些由神经肽调节。通过利用质谱 (MS)，本研究旨在提供蓝蟹 Callinectes sapidus 中神经肽的全局视角，以及由于急性、严重缺氧（类似于 10% O-2 水饱和度）应激而导致的神经肽随时间（0、1、4 和 8 小时）的变化，使用 4 重还原二甲基化策略来提高通量。这项研究同时使用电喷雾电离和基质辅助激光解吸/电离 (MALDI) MS，提供了互补的覆盖范围，从而能够鉴定 88 种神经肽。有趣的趋势包括 (1) 由于缺氧暴露导致神经肽表达总体下降，(2) 在初始反应后暴露 4 或 8 小时后恢复到基础水平，(3) 仅在暴露 4 小时以上后才发生变化，以及 (4) 振荡模式。总体而言，这项研究增强了多重定量的能力，以了解严重缺氧应激随时间推移造成的大规模变化。
Hypoxia (i.e., low oxygen (O-2) levels) is a common environmental challenge for several aquatic species, including fish and invertebrates. To survive or escape these conditions, these animals have developed novel biological mechanisms, some regulated by neuropeptides. By utilizing mass spectrometry (MS), this study aims to provide a global perspective of neuropeptides in the blue crab, Callinectes sapidus, and their changes over time (0, 1, 4, and 8 h) due to acute, severe hypoxia (similar to 10% O-2 water saturation) stress using a 4-plex reductive dimethylation strategy to increase throughput. Using both electrospray ionization and matrix-assisted laser desorption/ionization (MALDI) MS, this study provides complementary coverage, allowing 88 neuropeptides to be identified. Interesting trends include (1) an overall decrease in neuropeptide expression due to hypoxia exposure, (2) a return to basal levels after 4 or 8 h of exposure following an initial response, (3) changes only after 4+ h exposure, and (4) an oscillating pattern. Overall, this study boosts the power of multiplexed quantitation to understand the large-scale changes due to severe hypoxia stress over time.