GUANOSINE 5'-DIPHOSPHATE D-MANNOSE - GUANOSINE 5'-DIPHOSPHATE L-GALACTOSE EPIMERASE OF CHLORELLA-PYRENOIDOSA - CHEMICAL SYNTHESIS OF GUANOSINE 5'-DIPHOSPHATE 5'-DIPHOSPHATE L-GALACTOSE AND FURTHER-STUDIES OF THE ENZYME AND THE REACTION IT CATALYZES
GUANOSINE 5'-DIPHOSPHATE D-MANNOSE - GUANOSINE 5'-DIPHOSPHATE L-GALACTOSE EPIMERASE OF CHLORELLA-PYRENOIDOSA - CHEMICAL SYNTHESIS OF GUANOSINE 5'-DIPHOSPHATE 5'-DIPHOSPHATE L-GALACTOSE AND FURTHER-STUDIES OF THE ENZYME AND THE REACTION IT CATALYZES
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DOI：
10.1016/0003-9861(79)90644-1
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发表时间：
1979-01-01
影响因子：
3.9
通讯作者：
BARBER, GA
中科院分区：
文献类型：
--
作者：
HEBDA, PA;BEHRMAN, EJ;BARBER, GA
一种从绿色灵芝提取物中提取的酶。进一步纯化催化GDP D-甘露糖可逆差向异构化为GDP L-半乳糖的蛋白核酶。底物GDP L-半乳糖通过吗啉化过程化学制备。开发了一种改进的方法用于合成该方法中的中间体β- L-吡喃半乳糖磷酸，通过L-半乳糖的原酸酯。研究了该酶的各种特性及其催化的反应。本文介绍了一种新的方法，即用GLC跟踪未标记底物的反应过程。
An enzyme from extracts of the green alga C. pyrenoidosa that catalyzes the reversible epimerization of GDP D-mannose to GDP L-galactose was further purified. The substrate GDP L-galactose was made chemically by the morpholidate procedure. An improved method was developed for the synthesis of an intermediate in that process, .beta.-L-galactopyranosyl phosphate, via an orthoester of L-galactose. Various characteristics of the enzyme and the reaction it catalyzes were studied. A new method using GLC was introduced for following the course of the reaction with unlabeled substrates.