The segment polarity phenotype of Drosophila involves differential tendencies toward transformation and cell death.
The segment polarity phenotype of Drosophila involves differential tendencies toward transformation and cell death.
复制标题
果蝇的节段极性表型涉及转化和细胞死亡的不同倾向。
DOI：
10.1016/0012-1606(89)90084-5
复制
发表时间：
1989
影响因子：
2.7
通讯作者：
Perrimon,N
中科院分区：
文献类型：
--
作者：
Klingensmith,J;Noll,E;Perrimon,N
果蝇的节段极性基因是段内组织所必需的，突变胚胎中的角质层形态异常揭示了这一点。大多数这些部位的病变导致相似的角质层表型，正常情况下裸露的节段后部区域不同程度地被异位齿状物覆盖。一种温度敏感的蚂蚁等位基因，它允许我们改变ARM+的活动水平，产生了整个范围的表型，这表明这些基因影响着一条共同的途径。之前对ARM的一个强等位基因的研究表明，该基因座是细胞自主的，因为小的纯合子表皮克隆分泌牙齿。我们已经在所有水平的ARM+活性上进行了类似的克隆分析。这显示了片段内细胞转化和细胞死亡的不同趋势。对片段基因融合产物的抗体显示，细胞死亡主要在片段的最后面区域。我们认为，导致转化或死亡的不同细胞再特化参与了片段极性表型的产生。
The segment polarity genes ofDrosophilaare required for intrasegmental organization, as revealed by their abnormal cuticular morphology in mutant embryos. Lesions in most of these loci result in a similar cuticular phenotype, in which the normally naked, posterior region of the segment is covered to varying degrees by ectopic denticles. A temperature-sensitive allele ofarmadillo, which allows us to vary the level ofarm+activity, generates this entire range of phenotypes, suggesting that these genes affect a common pathway. Previous work with a strong allele ofarmrevealed the locus to be cell-autonomous, in that small homozygous epidermal clones secreted denticles. We have conducted a similar clonal analysis at all levels ofarm+activity. This shows a differential tendency toward cell transformation and cell death within the segment. Antibodies to segmentation gene-fusion products show that the cell death is primarily in the most posterior region of the segment. We suggest that differential cell respecification, resulting in transformation or death, is involved in generating the segment polarity phenotype.