Enhanced fluorescence of chitosan based on size change of micelles and application to directly selective detecting Fe3+ in human serum
Enhanced fluorescence of chitosan based on size change of micelles and application to directly selective detecting Fe3+ in human serum
复制标题
基于胶束尺寸变化的壳聚糖荧光增强及其在直接选择性检测人血清中Fe3中的应用
DOI：
10.1016/j.bios.2012.10.098
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发表时间：
2013-04-15
影响因子：
12.6
通讯作者：
Xia, Xiaodong
中科院分区：
文献类型：
--
作者：
Huang, Haowen;Liu, Fang;Xia, Xiaodong
在本文中，我们已经开发了一种方法，显着增强壳聚糖的荧光，通过简单地加热固有的低荧光壳聚糖水溶液。壳聚糖溶液的蓝色荧光增强是由于长时间加热后壳聚糖形成了小尺寸的胶束。当Fe 3+离子结合并从壳聚糖胶束中释放出来时，壳聚糖胶束的荧光被猝灭并恢复。因此，未修饰官能团的壳聚糖对Fe 3+具有很高的识别选择性。因此，一个新的荧光传感器，灵敏地检测Fe 3+离子的基础上的壳聚糖胶束尺寸的变化，随后制成。这种增强的荧光使壳聚糖传感器能够对低浓度的Fe 3+敏感，并且在1.96 × 10（-8）至2.00 × 10（-5）M的范围内具有线性响应。更重要的是，这种新型传感器可以应用于人血清中的Fe 3+离子的直接检测，而无需样品预处理。对5份人血清样品的分析表明，Fe ~（3+）的平均浓度为26.95 μ M，与其它方法测定结果一致。此外，基于壳聚糖的测定的优点是可以直接测量人血清中的Fe 3+而不是Fe 2+，避免了昂贵、耗时和复杂的过程。(C)2012爱思唯尔有限公司版权所有。
In this paper, we have developed an approach to significantly enhance fluorescence of chitosan by simply heated the inherently low fluorescent chitosan aqueous solution. Enhanced blue fluorescence of chitosan solution was observed as originated from the formation of small size of chitosan micelle after long time heated. The fluorescence of chitosan micelles was quenched and recovered when Fe3+ ions were combined and released from chitosan micelles. Therefore, chitosan without modification of functional groups can recognize Fe3+ with very high selectivity. As a result, a new fluorescence sensor for sensitively detecting Fe3+ ion based on the change of chitosan micelles sizes was subsequently fabricated. This enhanced fluorescence enables the chitosan sensor to be sensitive to low concentrations of Fe3+, and it is linear responsive in the range of 1.96 x 10(-8) to 2.00 x 10(-5) M. Importantly, this novel sensor may be applied in human serum for direct detection of Fe3+ ion without sample pretreatment. Analysis of 5 samples of human serum shows that the average concentration of Fe3+ is 26.95 mu M, which is consistent with the results determined by other methods. Moreover, the advantage of chitosan-based assay is that Fe3+ rather than Fe2+ in human serum can be directly measured, avoiding costly, time-consuming and complex process. (C) 2012 Elsevier B.V. All rights reserved.