Anti-inflammatory effects of 81 chinese herb extracts and their correlation with the characteristics of traditional chinese medicine.
Anti-inflammatory effects of 81 chinese herb extracts and their correlation with the characteristics of traditional chinese medicine.
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81种中药提取物的抗炎作用及其与中药特性的相关性
DOI：
10.1155/2014/985176
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Zhang DD
中科院分区：
文献类型：
--
作者：
Chen CL;Zhang DD
诱导型一氧化氮合酶(INOS)是一氧化氮过量产生的主要原因，其抑制剂作为有效的抗炎药一直被积极寻求。本研究从81种中草药中提取了70%的乙醇提取物。随后通过Griess反应和四甲基偶氮唑盐比色法评价了这些提取物对脂多糖/干扰素γ共刺激和未刺激的小鼠巨噬细胞RAW264.7细胞产生一氧化氮(NO)和细胞生长的影响。瑞香提取物、苏木提取物、毛冬青提取物、连翘提取物。Vahl、生姜、旋覆花、川芎对NO的产生有明显的抑制作用(100 μg/mL时抑制90%)。活性提取物(100 μg/mL50%抑制率)中，茜草、光果甘草、毛果、黑种草、野葛、葛根等植物活性成分的抑制率分别为：茜草、光果甘草、毛果甘草、野葛。DON对未刺激的RAW246.7细胞无细胞毒作用，但对脂多糖/干扰素γ共刺激细胞的生长有促进作用。通过分析其活性与其中药特性的相关性，发现具有辛辣味道的草药具有较强的抗炎作用。我们的研究提供了一系列潜在的抗炎药物，并提示具有辛辣味道的草药是有效抗炎药物的候选药物。
Inducible nitrogen oxide synthase (iNOS) is the primary contributor of the overproduction of nitric oxide and its inhibitors have been actively sought as effective anti-inflammatory agents. In this study, we prepared 70% ethanol extracts from 81 Chinese herbs. These extracts were subsequently evaluated for their effect on nitrogen oxide (NO) production and cell growth in LPS/IFNγ-costimulated and unstimulated murine macrophage RAW264.7 cells by Griess reaction and MTT assay. Extracts of Daphne genkwa Sieb.et Zucc, Caesalpinia sappan L., Iles pubescens Hook.et Arn, Forsythia suspensa (Thunb.) Vahl, Zingiber officinale Rosc, Inula japonica Thunb., and Ligusticum chuanxiong Hort markedly inhibited NO production (inhibition > 90% at 100 μg/mL). Among active extracts (inhibition > 50% at 100 μg/mL), Rubia cordifolia L., Glycyrrhiza glabra L., Iles pubescens Hook.et Arn, Nigella glandulifera Freyn et Sint, Pueraria lobata (Willd.) Ohwi, and Scutellaria barbata D. Don displayed no cytotoxicity to unstimulated RAW246.7 cells while increasing the growth of LPS/IFNγ-costimulated cells. By analyzing the correlation between their activities and their Traditional Chinese Medicine (TCM) characteristics, herbs with pungent flavor displayed potent anti-inflammatory capability. Our study provides a series of potential anti-inflammatory herbs and suggests that herbs with pungent flavor are candidates of effective anti-inflammatory agents.