Membrane properties of excitatory and inhibitory neurons in the rat prepositus hypoglossi nucleus
Membrane properties of excitatory and inhibitory neurons in the rat prepositus hypoglossi nucleus
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DOI：
10.1111/j.1460-9568.2008.06204.x
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发表时间：
2008-05
影响因子：
3.4
通讯作者：
M. Shino;S. Ozawa;N. Furuya;Yasuhiko Saito
中科院分区：
文献类型：
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作者：
M. Shino;S. Ozawa;N. Furuya;Yasuhiko Saito
舌下前核（PHN）被认为是一种神经结构，参与将眼睛或头部速度信号转化为水平眼球运动的眼睛位置信号。在本研究中，我们通过全细胞膜片钳记录，然后对大鼠脑干切片进行逆转录聚合酶链反应（RT-PCR）分析，研究了电生理膜特性与兴奋性和抑制性神经元细胞标记物表达模式之间的关系。每个神经元都具有三种类型的电压响应特性，即后超极化 (AHP)、放电模式和对超极化电流脉冲的响应。经过 RT-PCR 分析，我们将 PHN 神经元鉴定为谷氨酸能神经元 (n = 22) 或 GABA 能神经元 (n = 43)，尽管也鉴定出了少量胆碱能神经元 (n = 2) 和甘氨酸能神经元 (n = 1)。谷氨酸能和 GABA 能神经元都表现出多种膜特性；然而，我们发现了神经元类型和膜特性之间的一些特征关系。大多数神经元表现出（i）没有缓慢成分的AHP，（ii）第一个尖峰产生延迟的放电模式，（iii）具有瞬时爆发的放电模式和（iv）具有延长的初始放电间期的放电模式都是GABA能的。另一方面，谷氨酸能神经元的主要特征是放电率低。这些结果表明，某些类型的谷氨酸能和 GABA 能 PHN 神经元中特定的电生理膜特性与化学标记物的表达之间存在密切关系。
The prepositus hypoglossi nucleus (PHN) is thought to be a neural structure involved in transforming eye or head velocity signals into eye position signals for horizontal eye movements. In this study, we investigated the relationship between electrophysiological membrane properties and expression patterns of cellular markers for excitatory and inhibitory neurons by whole‐cell patch clamp recordings followed by reverse transcription polymerase chain reaction (RT‐PCR) analysis in rat brainstem slices. Three types of voltage response properties, namely afterhyperpolarization (AHP), firing pattern, and response to hyperpolarizing current pulses, were characterized in each neuron. Following RT‐PCR analysis, we identified PHN neurons as either glutamatergic (n = 22) or GABAergic (n = 43), although a small number of cholinergic (n = 2) and glycinergic neurons (n = 1) were also identified. Both glutamatergic and GABAergic neurons showed a wide variety of membrane properties; however, we found several characteristic relationships between neuronal type and membrane properties. Most neurons exhibiting (i) AHP without a slow component, (ii) a firing pattern with a delay in the generation of the first spike, (iii) a firing pattern with a transient burst and (iv) a firing pattern with a prolonged initial interspike interval were GABAergic. On the other hand, glutamatergic neurons were primarily characterized by a low firing rate. These results indicate that there is a close relationship between specific electrophysiological membrane properties and expression of chemical markers in some types of glutamatergic and GABAergic PHN neurons.