Studies on the human oocyte and its follicle. I. Ultrastructural and histochemical observations on the primordial follicle stage.
Studies on the human oocyte and its follicle. I. Ultrastructural and histochemical observations on the primordial follicle stage.
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DOI：
10.1083/jcb.34.2.647
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发表时间：
1967-08
影响因子：
7.8
通讯作者：
Adams, E C
中科院分区：
文献类型：
--
作者：
Hertig, A T;Adams, E C
成人卵巢原始（“静止”）卵泡中的卵母细胞含有复杂的副核结构，光学显微镜鉴定为巴尔比亚尼卵黄体。通过电子显微镜观察，这种结构由大量的线粒体和相关的内质网、围绕胞中心形成环状的多个复合聚集体以及附着在核膜上或游离在细胞质中的环状片层组成。复合聚集体包含液泡和精细划分的电子不透明材料。证据表明这种物质可能在卵母细胞与其环境之间运输。细胞中心包含一个无定形电子不透明沉积物的中央聚集体，这些沉积物似乎周期性地排列在细原纤维上，形成细胞中心外围的长粗纤维。在卵泡中期（雌激素）周期摘除的卵巢中，卵母细胞核膜附着或邻近环状片的明显流行表明，有必要进一步研究激素对静息卵母细胞的可能影响。光镜下在卵母细胞内未发现磷酸酶，但腺苷-单磷酸酶活性存在于原始卵泡周围的皮质细胞中，也存在于每个原始卵泡细胞的周围，最明显的是在卵母细胞一侧。葡萄糖-6-磷酸脱氢酶活性存在于卵母细胞细胞质内。
Oocytes in primordial ("resting") follicles in adult human ovaries contain a complex paranuclear structure identified by light microscopists as Balbiani's vitelline body. By electron microscopy this structure is composed of a mass of mitochondria with associated endoplasmic reticulum, multiple compound aggregates which form a ring around the cytocentrum, and a single stack or coil of annulate lamellae either attached to the nuclear membrane or free in the cytoplasm. The compound aggregates contain vacuoles and finely divided electron-opaque material. Evidence is presented for the probable transport of this material between the oocyte and its environment. The cytocentrum contains a central aggregate of amorphous electron-opaque deposits which appear to become periodically aligned on fine fibrils to form the long coarse fibers at the periphery of the cytocentrum. The apparent prevalence of annulate lamellae attached or adjacent to the nuclear membrane of oocytes in ovaries removed during the mid-follicular (estrogenic) phase of the cycle indicates the need for further study of a possible hormonal influence on the resting oocyte. By light microscopy phosphatases were not found within the oocyte, but adenosine-monophosphatase activity is present in the cortical cells surrounding primordial follicles, and also at the periphery of each primitive follicle cell, most prominently at the oocyte side. Glucose-6-phosphate dehydrogenase activity is present within the oocyte cytoplasm.