Use of a ROSA26:GFP transgenic line for long-term Xenopus fate-mapping studies
Use of a ROSA26:GFP transgenic line for long-term Xenopus fate-mapping studies
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DOI：
10.1111/j.1469-7580.2006.00608.x
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发表时间：
2006-09-01
影响因子：
2.4
通讯作者：
Marsh-Armstrong, Nicholas
中科院分区：
文献类型：
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作者：
Gross, Joshua B.;Hanken, James;Marsh-Armstrong, Nicholas
广泛和持久的标记表达是许多转基因应用，包括嵌合移植研究的先决条件。虽然现有的转基因工具爪蟾，非洲爪蟾，提供了一些启动子，驱动广泛的表达在胚胎阶段，获得通过变态和分化的成年组织的转基因表达已经很难实现与这个物种。在这里，我们报告了小鼠ROSA 26启动子在非洲爪蟾中的应用。GFP在变性后阶段检查的每种转基因组织和细胞类型中表达。此外，转基因ROSA 26：GFP青蛙发育正常，相对于野生型青蛙在生长或形态上没有明显差异。ROSA 26转基因可作为非洲爪蟾成体结构胚胎命运定位的可靠标记。这种转基因系的效用说明了它在嵌合移植研究，证明成人骨颌胚胎颅神经嵴的衍生。
Widespread and persistent marker expression is a prerequisite for many transgenic applications, including chimeric transplantation studies. Although existing transgenic tools for the clawed frog, Xenopus laevis, offer a number of promoters that drive widespread expression during embryonic stages, obtaining transgene expression through metamorphosis and into differentiated adult tissues has been difficult to achieve with this species. Here we report the application of the murine ROSA26 promoter in Xenopus. GFP is expressed in every transgenic tissue and cell type examined at post-metamorphic stages. Furthermore, transgenic ROSA26:GFP frogs develop normally, with no apparent differences in growth or morphology relative to wild-type frogs. ROSA26 transgenes may be used as a reliable marker for embryonic fate-mapping of adult structures in Xenopus laevis. Utility of this transgenic line is illustrated by its use in a chimeric grafting study that demonstrates the derivation of the adult bony jaw from embryonic cranial neural crest.