Cryolathe Corneal Injury
Cryolathe Corneal Injury
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冷冻角膜损伤
DOI：
10.1097/00003226-198302010-00007
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发表时间：
1983
期刊：
影响因子：
2.8
通讯作者：
E. P. Kay
中科院分区：
文献类型：
--
作者：
D. Schanzlin;J. Jester;E. P. Kay
采用兔板层角膜移植术（LKP）模型，我们研究了标准化Barraquer冷冻损伤对角膜结构和角膜细胞生物化学的影响。冷冻角膜冷冻损伤引起基质角膜细胞立即破裂，并引发炎症细胞反应，在冷冻损伤后3天达到峰值。冷冻的兔LKP角膜在术后早期是模糊的，其外观类似于人类角膜磨镶术和角膜镜片术病例的术后外观。临床表现与角膜细胞死亡和纤维间胶原距离增加相关。角膜细胞再生发生在21天内。再生的“成角膜细胞”的特征是活跃的线粒体和粗面内质网的增加。角膜中央的角膜细胞计数与角膜透明度显著相关。通过在冷冻LKP角膜中再生角膜基质细胞的胶原蛋白合成的特征在于，与通过新鲜LKP角膜合成的胶原蛋白相比，V型胶原蛋白相对于I型胶原蛋白的比例降低。虽然I型和V型胶原的胶原表型表达没有改变，但冷冻LKP角膜中新合成的胶原的溶解特性发生了变化，盐溶胶原显著减少。这一发现表明，再生角膜细胞合成的胶原蛋白更快地掺入成熟的胶原蛋白形式。这些研究表明，冷冻屈光手术过程中产生的冷冻损伤对角膜结构和角膜细胞生物化学产生了显着的早期影响。
Using a rabbit model of lamellar keratoplasty (LKP), we examined the effect of standardized Barraquer cryolathe freeze injury on corneal structure and keratocyte biochemistry. Cryolathe corneal freeze injury caused immediate disruption of the stromal keratocytes and initiated an inflammatory cell reaction peaking at 3 days following freeze injury. Frozen rabbit LKP corneas were hazy in the early postoperative period, an appearance analogous to the postoperative appearance of human keratomileusis and keratophakia cases. The clinical appearance correlated with keratocyte death and increased interfiber collagen distance. Keratocyte regeneration occurred within 21 days. The regenerating “keratoblasts” were characterized by active mitochondria and an increase in the rough endoplasmic reticulum. Keratocyte counts in the central cornea were found to correlate significantly with corneal clarity. Collagen synthesis by regenerating keratocytes in frozen LKP corneas was characterized by a decrease in the proportion of type V collagen relative to type I collagen when compared to that synthesized by fresh LKP corneas. Although the collagen phenotypic expression of type I and type V collagen was unaltered, there was a change in the solubility characteristics of newly-synthesized collagen in frozen LKP corneas, with a significant decrease in the salt-solubilized collagen. This finding suggests that the collagen synthesized by regenerating keratocytes was more quickly incorporated into mature collagen forms. These studies suggest that freeze injury produced by cryolathe refractive surgical procedures induces a significant early effect on corneal structure and keratocyte biochemistry.