Locus of the Lethal Event in the Serum Bactericidal Reaction
Locus of the Lethal Event in the Serum Bactericidal Reaction
复制标题
血清杀菌反应中致死事件的轨迹
作者：
D. Feingold;J. Goldman;H. Kuritz
高渗蔗糖抑制不含溶菌酶的血清对大肠杆菌粗株的杀菌活性。抑制的持续时间与蔗糖引起的质壁分解的持续时间相关。尽管血清的致死作用被推迟，但细胞迅速释放碱性磷酸酶表明，在这种条件下对细胞壁的非致命性损伤已经发生。相比之下，细胞对β-半乳糖苷酶的隐蔽性并没有恶化，直到细菌的活力开始下降。结论是，血清的主要作用部位是细菌细胞壁；然而，在没有溶菌酶的情况下，致死事件是随后对细菌细胞膜的破坏。
Hypertonic sucrose inhibited the bactericidal activity of lysozyme-free serum against a rough strain of Escherichia coli. The duration of the inhibition correlated with the duration of plasmolysis caused by the sucrose. Although the lethal action of the serum was delayed, the prompt release of alkaline phosphatase by the cells suggested that nonlethal damage to the cell wall had taken place under these conditions. In contrast, the crypticity of the cells for β-galactosidase did not deteriorate until the viability of the bacteria began to decrease. It is concluded that the primary site of action of serum is at the bacterial cell wall; however, in the absence of lysozyme, the lethal event was subsequent damage to the bacterial cell membrane.