Real-time manipulation of T cell-dendritic cell interactions in vivo reveals the importance of prolonged contacts for CD4+ T cell activation
Real-time manipulation of T cell-dendritic cell interactions in vivo reveals the importance of prolonged contacts for CD4+ T cell activation
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DOI：
10.1016/j.immuni.2007.08.018
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发表时间：
2007-10-01
期刊：
影响因子：
32.4
通讯作者：
Bousso, Philippe
中科院分区：
文献类型：
--
作者：
Celli, Susanna;Lemaitre, Fabrice;Bousso, Philippe
T细胞与树突状细胞（DC）相互作用的时间从几分钟到数小时不等。然而，这种相互作用的持续时间与T细胞活化效率之间的因果联系在体内尚未确定。通过活体双光子成像，我们实时操纵T细胞 - DC相互作用，发现T细胞与DC的首次相遇往往会导致长时间的相互作用。此外，T细胞受体 - 主要组织相容性复合体信号的终止促进了细胞分离，这表明DC上的抗原可利用性调节接触持续时间。最后，初始CD4(+) T细胞进行克隆扩增需要至少6小时的体内T细胞 - DC相互作用。这些结果确立了长时间的T细胞 - DC相互作用对于体内有效的CD4(+)细胞活化的重要性。
T cells interact with dendritic cells (DCs) for periods lasting from minutes to hours. However, a causal link between the duration of this interaction and the efficiency of T cell activation has not been established in vivo. Employing intravital two-photon imaging, we manipulated T cell-DC interactions in real time and found that the first T cell-DC encounter often resulted in a long-lived interaction. Moreover, the cessation of T cell receptor-major histocompatibility complex signals promoted cellular dissociation, suggesting that antigen availability on DCs regulates contact duration. Finally, at least 6 hr of in vivo T cell-DC interaction were required for naive CD4(+) T cells to undergo clonal expansion. These results establish the importance of prolonged T cell-DC interactions for efficient CD4(+) cell activation in vivo.