Preparation of 3-D regenerated fibroin scaffolds with freeze drying method and freeze drying/foaming technique
Preparation of 3-D regenerated fibroin scaffolds with freeze drying method and freeze drying/foaming technique
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DOI：
10.1007/s10856-006-0610-z
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发表时间：
2006-12-01
影响因子：
3.7
通讯作者：
Feng, QingLing
中科院分区：
文献类型：
--
作者：
Lv, Qiang;Feng, QingLing
虽然用冷冻干燥方法制备了三维丝蛋白支架，但其孔隙率和孔径仍不能满足组织工程的要求。本文通过调节丝素浓度，首次采用冷冻干燥的方法制备了高孔隙率、直径为100 μ m的丝素蛋白多孔支架。用扫描电镜观察了不同纤维蛋白浓度冻干后支架的形态。并提出了一种新的冷冻干燥改进方法——冷冻干燥/发泡技术，用于制备不同浓度的丝素蛋白支架。采用该方法，12%浓度制备的纤维蛋白支架的孔隙率和孔径分别为85.8 +/- 4%和109 +/- 20 μ m，屈服强度可达450 +/- 6 KPa； 8%浓度制备的纤维蛋白支架的孔隙率和孔径分别为96.9 +/- 3.6%和120 +/- 30 μ m，屈服强度可达30 +/- 1 KPa。冷冻干燥/发泡技术生产的支架具有高屈服强度、相互连接的孔隙和孔径大于100 μ m的有用组合。通过调节丝素蛋白浓度和解冻时间，可以控制其孔隙率、孔径大小和力学性能，以满足组织工程的不同要求。结果表明，采用上述方法制备的丝蛋白支架在生物材料领域具有广泛的应用前景。
Although three-dimensional fibroin scaffolds have been prepared with freeze drying method, the porosity and pore sizes still can not satisfy the requirement of tissue engineering. In this article, fibroin porous scaffold with high porosity and > 100 mu m diameter interconnected pores was firstly prepared with freeze drying method through adjusting fibroin concentration. The morphology of different scaffolds lyophilized from different fibroin concentration was observed by SEM. A novel freeze drying improved method, freeze drying/foaming technique, was also devised to prepare fibroin scaffolds at different fibroin concentrations. Using the said method, the porosity and pore size of fibroin scaffolds prepared from 12% concentration were 85.8 +/- 4% and 109 +/- 20 mu m respectively with yield strength up to 450 +/- 6 KPa while the porosity and pore size of fibroin scaffolds prepared from 8% concentration were 96.9 +/- 3.6% and 120 +/- 30 mu m respectively with yield strength up to 30 +/- 1 KPa. The freeze drying/foaming technique produced scaffolds with a useful combination of high yield strength, interconnected pores, and pore sizes greater than 100 mu m in diameter. Through adjusting fibroin concentration and thawing time, the porosity, pore sizes and mechanical properties could be controlled to satisfy the different requirements of tissue engineering. The results suggested that fibroin scaffolds prepared with the above methods could be formed for utility in biomaterial application.