Differential brain-derived neurotrophic factor and transforming growth factor-beta expression in the rat cochlea following deafness.
Differential brain-derived neurotrophic factor and transforming growth factor-beta expression in the rat cochlea following deafness.
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耳聋后大鼠耳蜗中脑源性神经营养因子和转化生长因子-β 表达的差异。
DOI：
10.1097/01.wnr.0000233088.92839.23
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Stover,Timo
中科院分区：
文献类型：
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作者：
Wissel,Kirsten;Wefstaedt,Patrick;Miller,JosefM;Lenarz,Thomas;Stover,Timo
脑源性神经营养因子和转化生长因子-β亚型在耳蜗功能中具有重要作用。本研究采用逆转录聚合酶链式反应和免疫组织化学方法，检测正常听力和耳聋大鼠听神经和下丘中脑源性神经营养因子、转化生长因子β1/2及其主要受体TrkB和转化生长因子βR1/R2的基因和蛋白表达模式。耳蜗注射新霉素(10%)致聋。耳聋后下丘基因表达无明显变化，而听神经中BDNFmRNA、转化生长因子β1和转化生长因子βR1mRNA表达上调。此外，毛细胞脱失后，BDNF蛋白和细胞因子在听神经中表达。提示脑源性神经营养因子和转化生长因子β-1作为内源性生存因子的重要性。
Brain derived neurotrophic factor (BDNF) and transforming growth factor-β (TGFβ) subtypes have demonstrated their importance in cochlear functions. The aim of this study was to determine gene and protein expression patterns of BDNF, TGFβ1/2 and their main receptors trkB and TGFβR1/R2 in the auditory nerve and inferior colliculus of normal hearing and deafened rats by reverse-transcriptase polymerase chain reaction and immunohistochemistry. Deafening was performed by cochlear injection of neomycin (10%). Significant gene expression changes were not found in the inferior colliculus after deafness; however, in the auditory nerve, BDNF, TGFβ1 and TGFβR1 mRNA in particular were upregulated. Additionally, BDNF protein and the cytokines were found to be expressed in the auditory nerve after hair cell loss. These data indicate the importance of BDNF and TGFβ1 as endogenous survival factors.