Natural products from isnA-containing biosynthetic gene clusters recovered from the genomes of cultured and uncultured bacteria
Natural products from isnA-containing biosynthetic gene clusters recovered from the genomes of cultured and uncultured bacteria
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DOI：
10.1021/ja075492v
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发表时间：
2007-10-10
影响因子：
15
通讯作者：
Daniels, Rachel
中科院分区：
文献类型：
--
作者：
Brady, Sean F.;Bauer, John D.;Daniels, Rachel
异腈合酶isnA的序列被用于搜索完全测序的细菌基因组以及直接从环境样品中提取的DNA，以寻找可能在新的天然产物的生物合成中使用isnA同系物的操纵子。这份手稿描述了所发现的生物合成操纵子和由这些操纵子的异源表达产生的化合物。从这个操纵子家族的表达中鉴定出新的代谢物表明，即使是简单的、测序的天然产物生物合成基因簇的激活，使用标准的E。大肠杆菌蛋白表达载体将是发现结构新颖的代谢物的有益和直接的策略。
The sequence of an isonitrile synthase, isnA, was used to search fully sequenced bacterial genomes as well as DNA extracted directly from environmental samples for operons that might use isnA homologues in the biosynthesis of new natural products. This manuscript describes the biosynthetic operons that were found and the compounds that were produced from the heterologous expression of these operons. The identification of new metabolites from the expression of this family of operons suggests that the activation of even simple, sequenced natural product biosynthetic gene clusters using standard E. coli protein expression vectors will be a rewarding and straightforward strategy for the discovery of structurally novel metabolites.