In vivo nitric oxide sensor using non-conducting polymer-modified carbon fiber.
In vivo nitric oxide sensor using non-conducting polymer-modified carbon fiber.
复制标题
DOI：
10.1016/s0956-5663(98)00078-5
复制
发表时间：
1998-11
影响因子：
12.6
通讯作者：
J. -. Park;P. H. Tran;J. K. Chao;R. Ghodadra;R. Rangarajan;N. Thakor
中科院分区：
文献类型：
--
作者：
J. -. Park;P. H. Tran;J. K. Chao;R. Ghodadra;R. Rangarajan;N. Thakor
一氧化氮（NO）是一种非常重要的、普遍存在于体内的气体信使。研究了由非导电聚合物改性碳纤维电极制成的NO传感器的响应特性，以确定其在体内使用的选择性、灵敏度和稳定性。由Nafion、间苯二胺和间苯二酚组成的复合聚合物对NO的安培检测具有良好的选择性和稳定性。这种不导电、自限制的聚合物薄膜保护电极不受其他生物化学物质的干扰和污染。虽然改性后的传感器对NO的相对灵敏度低于未改性碳纤维电极（小于6%），但复合聚合物电极对抗坏血酸（>2000:1）、亚硝酸盐（>600:1）和多巴胺（>200:1）具有较高的选择性。NO传感器的稳定性维持至少1周。体内实验后NO敏感性（n=8）为体内实验前初始敏感性数据的88.1±5.6%。用这种电极进行的初步体内实验显示，在缺血性损伤后，大脑中一氧化氮水平升高。
Nitric oxide (NO) is emerging as a very important and ubiquitous gaseous messenger in the body. The response characteristics of NO sensors made of non-conducting polymer modified carbon fiber electrodes are investigated to determine their selectivity, sensitivity, and stability for in vivo use. A composite polymer, comprising Nafion, m-phenylenediamine, and resorcinol, showed the best selectivity and stability to amperometric NO detection. The non-conducting, self-limiting polymer film protects the electrode from interference and fouling by other biochemicals. Although the relative sensitivity to NO of the modified sensor is lower than that of the unmodified carbon fiber electrodes (less than 6%), the composite polymer electrode showed high selectivity against ascorbic acid (>2000:1), nitrite (>600:1), and dopamine (>200:1). The stability of the NO sensor was maintained for at least 1 week. The NO sensitivity after in vivo experiments (n=8) is 88.1±5.6% of initial sensitivity data obtained before in vivo experiments. Preliminary in vivo experiments done with this electrode are shown to capture elevated NO levels in brain following an ischemic injury.