MULTIPLE SEQUENCE ELEMENTS IN THE C-FOS PROMOTER MEDIATE INDUCTION BY CAMP
MULTIPLE SEQUENCE ELEMENTS IN THE C-FOS PROMOTER MEDIATE INDUCTION BY CAMP
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DOI：
10.1101/gad.3.2.198
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发表时间：
1989-02-01
影响因子：
10.5
通讯作者：
ROEDER, RG
中科院分区：
文献类型：
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作者：
FISCH, TM;PRYWES, R;ROEDER, RG
cAMP对NIH-3 T3细胞c-fos的诱导作用与血清的诱导作用不同。血清诱导是由血清反应元件（SRE）介导的，而cAMP诱导则不需要该元件。事实上，c-fos启动子/增强子中没有一个序列元件是cAMP应答所必需的。相反，c-fos启动子/增强子中的多个序列元件可以独立地介导cAMP的诱导。这些要素是：（1）从-72到-54的区域，其含有细胞活化转录因子（ATF）的结合位点;（2）从-225到-99的区域;（3）从-303到-281的区域，其与转录因子AP 1的共有结合位点同源;（4）-317 ~-298区域，包含SRE。这些序列元件在与cAMP不响应的“最小"fos启动子（-53至+42）融合时传递cAMP诱导。此外，c-fos区域从-700到-63和从-71到-48可以赋予cAMP对异源启动子的诱导。
Induction of c-fos by cyclic AMP in NIH-3T3 cells is distinct from induction by serum. Whereas induction by serum is mediated by the serum response element (SRE), induction by cAMP does not require this element. In fact, no single sequence element in the c-fos promoter/enhancer is stringently required for the cAMP response. Rather, multiple sequence elements in the c-fos promoter/enhance can mediate induction by cAMP independently. These elements are: (1) the region from -72 to -54, which contains a binding site for a cellular activating transcription fator (ATF); (2) the region from -225 to -99; (3) the region from -303 to -281, which is homologous to the consensus binding site for the transcription factor AP1; and (4) the region from -317 to -298, which contains the SRE. These sequence elements convey cAMP inducibility when fused to the cAMP-inresponsive ''minimal'' fos promoter (-53 to +42). In addition, the c-fos regions from -700 to -63 and from -71 to -48 can confer cAMP inducibility to a heterologous promoter.