SpTIA-1 suppresses WSSV infection by promoting apoptosis in mud crab (Scylla paramamosain)
SpTIA-1 suppresses WSSV infection by promoting apoptosis in mud crab (Scylla paramamosain)
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SpTIA-1 通过促进青蟹 (Scylla paramamosain) 细胞凋亡来抑制 WSSV 感染
DOI：
10.1016/j.molimm.2021.10.015
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发表时间：
2021-10-27
影响因子：
3.6
通讯作者：
Gong, Yi
中科院分区：
文献类型：
--
作者：
Hu, Hang;Zhao, Xinshan;Gong, Yi
TIA-1（T细胞限制性细胞内抗原-1）是一种RNA结合蛋白，其作为CED-9（BCL 2同源物）的下游，在应激条件下诱导细胞凋亡。迄今为止，TIA-1介导的细胞凋亡在高等动物中的作用已被广泛研究，而细胞凋亡恰好与生物免疫防御有关。然而，TIA-1参与病毒感染期间免疫功能的研究尚未得到明确的研究，特别是在海洋无脊椎动物中。本研究对拟穴青蟹（Scylla paramamosain）体内的SpTIA-1进行了特异性鉴定。SpTIA-1的开放阅读框架（ORF）由1116个碱基组成，编码372个氨基酸。另外，SpTIA-1在白色斑点综合征病毒（WSSV）感染的青蟹血细胞中的表达明显上调。此外，通过RNAi方法，我们发现SpTIA-1可以激活Caspase-3信号通路，增加ROS水平，降低线粒体膜电位，导致血细胞凋亡率增加，最终抑制WSSV在青蟹体内的增殖。因此，本研究提高了对青蟹抗病毒免疫的认识，并为海洋甲壳动物的天然免疫提供了新的见解。
TIA-1 (T cell restricted intracellular antigen-1) is a kind of RNA-binding protein which serves as the downstream of CED-9 (a BCL2 homolog) and induces apoptosis under stress conditions. So far, the function of apoptosis mediated by TIA-1 has been extensively studied in higher animals, and apoptosis happens to be related to biological immune defense. However, the involvement of TIA-1 in the study of immune function during viral infection has not been clearly studied, especially in marine invertebrates. In the study, SpTIA-1 in mud crab (Scylla paramamosain) was specifically identified. The Open Reading Frame (ORF) of SpTIA-1 was consisted of 1116 nucleotide bases and encoded 372 amino acids. Besides, the results showed that the expression of SpTIA-1 was obviously up-regulated during WSSV (White Spot Syndrome Virus) infection in hemocytes of mud crab. Furthermore, through RNAi approach, we found that SpTIA-1 could activate Caspase-3 signaling and increase ROS levels to reduce mitochondrial membrane potential, resulting in the increase of apoptosis rate in hemocytes, which eventually suppressed WSSV multiplication in mud crab. The current study therefore improves the knowledge of antiviral immunity in mud crab and provides new insights into the innate immunity of marine crustaceans.