Proteins containing the UBA domain are able to bind to multi-ubiquitin chains
Proteins containing the UBA domain are able to bind to multi-ubiquitin chains
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DOI：
10.1038/ncb1001-939
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发表时间：
2001-10-01
影响因子：
21.3
通讯作者：
Gordon, C
中科院分区：
文献类型：
--
作者：
Wilkinson, CRM;Seeger, M;Gordon, C
UBA结构域是在多种蛋白质中发现的基序，其中一些与泛素-蛋白酶体系统有关（1,2）。我们描述了分裂酵母基因mud1（+）的分离，该基因编码含有能够结合多泛素链的蛋白的UBA结构域。我们展示了UBA域负责此活动。另外两种含有该基序的蛋白，即Rad23和Dsk2的分裂酵母同源物，也被证明通过其UBA结构域结合多泛素链。这两种蛋白与26s蛋白酶体的分裂酵母Pus1（S5a/Rpn10）亚基一起参与了该蛋白水解复合物对底物的识别和周转。
The UBA domain is a motif found in a variety of proteins, some of which are associated with the ubiquitin-proteasome system(1,2). We describe the isolation of a fission-yeast gene, mud1(+), which encodes a UBA domain containing protein that is able to bind multi-ubiquitin chains. We show that the UBA domain is responsible for this activity. Two other proteins containing this motif, the fission-yeast homologues of Rad23 and Dsk2, are also shown to bind multi-ubiquitin chains via their UBA domains. These two proteins are implicated, along with the fission-yeast Pus1(S5a/Rpn10) subunit of the 26 S proteasome, in the recognition and turnover of substrates by this proteolytic complex.