Synthesis, characterization and chondrocyte culture of polyhedral oligomeric silsesquioxane (POSS)-containing hybrid hydrogels
Synthesis, characterization and chondrocyte culture of polyhedral oligomeric silsesquioxane (POSS)-containing hybrid hydrogels
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含有多面体低聚倍半硅氧烷（POSS）的杂化水凝胶的合成、表征和软骨细胞培养
DOI：
10.1039/c5ra27989e
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发表时间：
2016-01-01
期刊：
影响因子：
3.9
通讯作者：
Zhang, Weian
中科院分区：
文献类型：
--
作者：
Pan, Xiuwei;Gao, Hao;Zhang, Weian
通过八叠氮基倍半硅氧烷（OAPOSS）与炔基聚乙二醇（PEG）的快速叠氮-炔键反应，成功制备了多面体倍半硅氧烷（POSS）杂化水凝胶。制备了一系列具有高度有序孔结构的POSS-PEG杂化水凝胶，通过改变PEG链长为3 k、6 k和10 k，可以很好地控制其孔径。POSS-PEG水凝胶在磷酸盐缓冲液（PBS）中浸泡后，表现出良好的吸水性能，其溶胀率随PEG链长的增加而增大。流变学测量表明，所有的水凝胶表现出弹性体的特性。弹性模量和极限应力（断裂）显着提高与PEG链长的增加，这已被揭示的应力-应变力学分析。体外细胞培养结果表明，POSS-PEG（10 k）水凝胶均能很好地支持软骨细胞的贴壁、铺展和增殖，尤其是POSS-PEG（10 k）水凝胶。因此，POSS-PEG水凝胶显示了软骨组织工程的潜力。
Polyhedral oligomeric silsesquioxanes (POSS)-based hybrid hydrogels were successfully prepared via a fast azide-alkyne click reaction between octa-azido-functionalized POSS (OAPOSS) and alkyne-functionalized poly(ethylene glycol) (PEG). A series of hybrid POSS-PEG hydrogels with the highly ordered porous structures were achieved and the pore size could be well controlled by varying the PEG chain length to 3k, 6k and 10k. After immersing in phosphate buffered saline (PBS), POSS-PEG hydrogels exhibited a good water absorption capacity, and their swelling ratio increased with the increase of the PEG chain length. Rheological measurements indicated that all hydrogels exhibited the characteristics of an elastomer. The elastic modulus and ultimate stress (at break) were significantly enhanced with the increase of the PEG chain length which has been revealed by stress-strain mechanical analysis. In vitro cell culture showed that all POSS-PEG hydrogels could well support chondrocytes attachment, spreading and proliferation, especially for the POSS-PEG (10k) hydrogel. Thus, POSS-PEG hydrogels exhibited the potential for cartilage tissue engineering.