Alteration of pectoral fin nerves following ablation of fin buds and by ectopic fin buds in the Japanese medaka fish.
Alteration of pectoral fin nerves following ablation of fin buds and by ectopic fin buds in the Japanese medaka fish.
复制标题
日本青鳉鱼鳍芽消融和异位鳍芽后胸鳍神经的改变。
DOI：
10.1016/0012-1606(91)90446-a
复制
发表时间：
1991
影响因子：
2.7
通讯作者：
Kuwada,JY
中科院分区：
文献类型：
--
作者：
Okamoto,H;Kuwada,JY
胸鳍芽的鳍轴突生长的胸鳍芽的作用进行了评估消融胸鳍芽和移植的鳍芽异位网站在胚胎中的日本青鳉鱼（Oryzias latipes）。S1 - 4节段神经在轴肌腹侧面向鳍芽方向延伸，在鳍芽基部汇合，S5节段神经较少。Okamoto和J. Y. Kuwada，1991，Dev. 146）。在运动生长锥开始侧向延伸之前，鳍芽消融后，S1-5中的神经沿着平行于同色异谱排列的轴肌的边界的每个节段的中间的轨迹而不是会聚。这一轨迹与不向鳍芽会聚的更多后段神经的轨迹相似。当鳍芽移植到较后节段时，S1-5的神经往往改变其轨迹并延伸到异位芽的基部。此外，运动神经从节段后S5，通常不支配的鳍芽，也延伸到异位鳍芽。当同时面对宿主和异位鳍芽时，运动神经延伸到任一鳍芽或分支并延伸到两个鳍芽。这些结果表明，早期的鳍芽是必要的鳍神经的正确的生长，并建议，鳍芽通常吸引鳍神经到其基础。鳍芽吸引运动生长锥的一个可能机制是鳍芽发出的长距离提示。
The role of the pectoral fin bud for outgrowth by fin axons was assessed by ablation of pectoral fin buds and by transplantation of fin buds to ectopic sites in the embryos of the Japanese medaka fish (Oryzias latipes). Normally nerves from segments 1–4 (S1–4) and less frequently the S5 nerve converged at the base of the fin bud by extending toward the fin bud on the ventral surface of the axial muscles (H. Okamoto and J. Y. Kuwada, 1991,Dev. Biol.146). Following ablation of the fin bud before motor growth cones have begun to extend laterally, nerves in S1–5 followed a trajectory down the middle of each segment parallel to the borders of the metamerically arranged axial muscles rather than converging. This trajectory was similar to that of more posterior segmental nerves which do not converge toward the fin bud. When fin buds were transplanted to more posterior segments, nerves from S1–5 often changed their trajectories and extended to the base of ectopic buds. Furthermore, motor nerves from segments posterior to S5, which normally do not innervate the fin bud, also extended to the ectopic fin bud. When faced with both the host and ectopic fin bud, motor nerves extended to either fin bud or branched and extended to both fin buds. These results demonstrate that the early fin bud is necessary for correct outgrowth of fin nerves and suggest that the fin bud normally attracts fin nerves to its base. One possible mechanism for the attraction of motor growth cones by the fin bud is a long distance cue emitted by the fin bud.