A single phosphatase can convert a robust step response into a graded, tunable or adaptive response
A single phosphatase can convert a robust step response into a graded, tunable or adaptive response
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DOI：
10.1099/mic.0.066324-0
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发表时间：
2013-07-01
期刊：
影响因子：
2.8
通讯作者：
Wadhams, George H.
中科院分区：
文献类型：
--
作者：
Chang, Yo-Cheng;Armitage, Judith P.;Wadhams, George H.
许多生物信号传导途径已经进化为对环境信号产生响应，这些信号对蛋白质拷贝数和噪音的波动具有鲁棒性。虽然对生物学有益，但这种鲁棒性对于希望重新设计并随后调整给定系统的响应的合成生物学家来说可能是有问题的。在这里，我们表明，良好的特点EnvZ/OmpR双组分信号系统，大肠杆菌具有这样一个强大的阶跃响应。然而，在系统中添加一种单独的成分，一种额外的独立可控的磷酸酶，可以改变这种行为，使其变得分级和可调，甚至显示出适应性。我们的方法引入了一种新的设计原理，可以简单地在工程和重新设计合成生物学的快速信号转导通路中实现。
Many biological signalling pathways have evolved to produce responses to environmental signals that are robust to fluctuations in protein copy number and noise. Whilst beneficial for biology, this robustness can be problematic for synthetic biologists wishing to re-engineer and subsequently tune the response of a given system. Here we show that the well-characterized EnvZ/OmpR two-component signalling system from Escherichia coli possesses one such robust step response. However, the synthetic addition of just a single component into the system, an extra independently controllable phosphatase, can change this behaviour to become graded and tunable, and even show adaptation. Our approach introduces a new design principle which can be implemented simply in engineering and redesigning fast signal transduction pathways for synthetic biology.