Inhibition by ethanol of forskolin-stimulated adenylate cyclase in a murine neuroblastoma clone (N1E-115).
Inhibition by ethanol of forskolin-stimulated adenylate cyclase in a murine neuroblastoma clone (N1E-115).
复制标题
乙醇对小鼠神经母细胞瘤克隆 (N1E-115) 中毛喉素刺激的腺苷酸环化酶的抑制。
DOI：
10.1016/0006-2952(85)90226-6
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发表时间：
1985
影响因子：
5.8
通讯作者：
Richelson,E
中科院分区：
文献类型：
--
作者：
Stenstrom,S;Seppala,M;Pfenning,M;Richelson,E
毛喉素，腺苷酸环化酶的二萜激活剂，刺激在完整的小鼠神经母细胞瘤克隆N1 E-115细胞的环AMP的形成，并刺激腺苷酸环化酶活性，从这些细胞的膜制备。乙醇引起的浓度依赖性抑制毛喉素刺激的反应，在这两种制剂。在完整细胞中，抑制似乎是非竞争性的。然而，在膜制剂中，抑制更多的是竞争性的。此外，两个系统中的抑制量也存在很大差异。因此，乙醇的抑制作用几乎是完整细胞膜的两倍。蔗糖似乎模仿乙醇的这些作用，表明对于完整细胞，这种醇的作用可能部分是由于细胞渗透压的变化。
Forskolin, a diterpene activator of adenylate cyclase, stimulated the formation of cyclic AMP in intact murine neuroblastoma clone N1E-115 cells and stimulated adenylate cyclase activity in a membranal preparation from these cells. Ethanol caused a concentration-dependent inhibition of the forskolin-stimulated responses in both preparations. In intact cells, the inhibition appeared to be noncompetitive. However, in the membranal preparation the inhibition was more of a competitive nature. In addition, there was also a large difference in the amount of inhibition in the two systems. Thus, the inhibition by ethanol was nearly twice as much with intact cells as with membranes. Sucrose appeared to mimic these effects of ethanol, suggesting that with intact cells the effect of this alcohol may be due, in part, to changes in cellular osmotic pressure.