Mapping quantitative trait loci for seed size traits in soybean (Glycine max L. Merr.)
Mapping quantitative trait loci for seed size traits in soybean (Glycine max L. Merr.)
复制标题
绘制大豆种子大小性状的数量性状位点 (Glycine max L. Merr.)
DOI：
10.1007/s00122-010-1471-x
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发表时间：
2011-02-01
影响因子：
5.4
通讯作者：
Zhang, Yuan-Ming
中科院分区：
文献类型：
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作者：
Xu, Yu;Li, He-Nan;Zhang, Yuan-Ming
大豆种子的大小性状（长、宽、厚）及其相应的长宽比、长宽比和宽厚比对种子的外观、品质和产量起着至关重要的作用。本研究以丽水种子黄与南农493-1正反交的F-2：3、F-2：4和F-2：5群体为材料，采用多QTL联合分析（MJA）沿着复合区间作图（CIM）的方法，对上述种子大小性状进行了QTL定位。共检测到121个主效QTL（M-QTL）、6个环境效应QTL、8个环境-QTL互作QTL、5个细胞质效应QTL和92个细胞质-QTL互作QTL。52个M-QTL在MJA和CIM间共有，21个M-QTL在2个以上群体间共有，5个M-QTL在3个群体间均有分布，表明M-QTL定位结果稳定。有5个M-QTL的遗传力较高，均大于20%。此外，CIM还证实了28个细胞质-QTL和4个环境-QTL互作。多数M-QTL分布在8个染色体区域。本研究结果为大豆籽粒大小性状相关基因的精细定位、克隆和分子设计育种奠定了基础。
Seed size traits in soybean-length, width and thickness-and their corresponding ratios-length-to-width, length-to-thickness and width-to-thickness-play a crucial role in determining seed appearance, quality and yield. In this study, an attempt was made to detect quantitative trait loci (QTL) for the aforementioned seed size traits in F-2:3, F-2:4 and F-2:5 populations from the direct and reciprocal crosses of Lishuizhongzihuang with Nannong 493-1, using multi-QTL joint analysis (MJA) along with composite interval mapping (CIM). A total of 121 main-effect QTL (M-QTL), six environmental effects, eight environment-by-QTL interactions, five cytoplasmic effects and 92 cytoplasm-by-QTL interactions were detected. Fifty-two common M-QTL across MJA and CIM, 21 common M-QTL in more than two populations and 5 M-QTL in all three populations showed the stability of the results. Five M-QTL had higher heritability, greater than 20%. In addition, 28 cytoplasm-by-QTL and 4 environment-by-QTL interactions were confirmed by CIM. Most M-QTL were clustered in eight chromosomal regions. Our results provide a good foundation for fine mapping, cloning and designed molecular breeding of favorable genes related to soybean seed size traits.