Neuronal regeneration in the cerebellum of adult teleost fish, Apteronotus leptorhynchus:: guidance of migrating young cells by radial glia
Neuronal regeneration in the cerebellum of adult teleost fish, Apteronotus leptorhynchus:: guidance of migrating young cells by radial glia
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DOI：
10.1016/s0165-3806(01)00193-6
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发表时间：
2001-09-23
期刊：
影响因子：
--
通讯作者：
Zupanc, GKH
中科院分区：
文献类型：
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作者：
Clint, SC;Zupanc, GKH
与哺乳动物相比，成年鱼表现出巨大的潜力，可以用新产生的神经元代替受伤的大脑神经元。本研究通过对纤维酸性蛋白（GFAP）的免疫染色鉴定放射状胶质细胞在神经元再生过程中的作用。机械损伤后约8天，与较短的存活时间相比，同侧背侧分子层放射状胶质纤维的面密度显著增加，或与完整大脑或损伤对侧半球的密度相比。在长达100天的调查期间，这一密度一直居高不下。纤维密度增加后，大约2天后，同侧背侧分子层的年轻细胞面密度增加，其特征是用核染料DAPI标记。基于这种显著的时空相关性，以及经常观察到的细长的年轻细胞与放射状胶质纤维的紧密结合，我们假设放射状胶质在引导年轻细胞从增殖区迁移到发生再生的病变部位方面发挥了重要作用。(C) 2001 Elsevier Science B.V.版权所有
In contrast to mammals, adult fish exhibit an enormous potential to replace injured brain neurons by newly generated ones. In the present study, the role of radial glia, identified by immunostaining against fibrillary acidic protein (GFAP), was examined in this process of neuronal regeneration. Approximately 8 days after application of a mechanical lesion to the corpus cerebelli in the teleost fish Apteronotus leptorhynchus, the areal density of radial glial fibers increased markedly in the ipsilateral dorsal molecular layer compared to shorter survival times, or to the densities found in the intact brain or in the hemisphere contralateral to the lesion. This density remained elevated throughout the time period of up to 100 days examined. The increase in fiber density was followed approximately 2 days later by a rise in the areal density of young cells, characterized by labeling with the nuclear dye DAPI, in the ipsilateral dorsal molecular layer. Based on this remarkable spatio-temporal correlation, and the frequently observed close apposition of elongated young cells to radial glial fibers, we hypothesize that radial glia play an important role in the guidance of migrating young cells from their proliferation zones to the site of lesion where regeneration takes place. (C) 2001 Elsevier Science B.V. All rights reserved.