Highly asymmetric intergeneric nuclear hybrids between Nicotiana and Petunia: evidence for recombinogenic and translocation events in somatic hybrid plants after “gamma”-fusion
Highly asymmetric intergeneric nuclear hybrids between Nicotiana and Petunia: evidence for recombinogenic and translocation events in somatic hybrid plants after “gamma”-fusion
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烟草和矮牵牛之间高度不对称的属间核杂种：“γ”融合后体细胞杂种植物中重组和易位事件的证据
DOI：
10.1007/bf00226798
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发表时间：
1991
影响因子：
5.4
通讯作者：
M. Jacobs
中科院分区：
文献类型：
--
作者：
S. Hinnisdaels;L. Bariller;A. Mouras;V. Sidorov;J. Del;J. Veuskens;I. Negrutiu;M. Jacobs
通过属间组合中的原生质体融合获得了极其不对称的核杂交体。将经辐射（钴 60,100 krad）卡那霉素抗性矮牵牛杂交叶肉原生质体与白花烟草野生型叶肉原生质体进行化学融合。在含有卡那霉素的培养基上选择了 86 个杂交菌落，其中 24 个可被诱导再生大量芽。再生体的细胞学分析表明，在一些品系中存在一些染色体片段，甚至在另一个品系中存在中间着丝粒染色体。除了额外的染色体片段之外，一些品系仅具有典型的烟草染色体，并且是二倍体（2n = 2X = 20）和四倍体（2n = 2X = 40）水平。生化分析表明，所有再生体都具有新霉素磷酸转移酶活性（NPTII），这表明基因组间重组和/或易位事件至少发生在那些无法检测到额外染色体片段的品系中。 Southern杂交表明NPTII基因的存在。所有测试的再生体都是可育的，并且一些再生体的卡那霉素基因（用于自花授粉和回交授粉至受体伴侣）的分离比率符合单基因显性标记的孟德尔规则。大多数再生体表现出异常的分离率；在这种情况下，分离率和染色体组成之间不能建立相关性。我们的结果表明，通过“γ”融合获得的核体细胞杂交植物中存在基因组间重组和易位事件。
Extremely asymmetric nuclear hybrids have been obtained via protoplast fusion in an intergeneric combination. Irradiated (cobalt60,100 krad) kanamycinresistantPetunia hybridamesophyll protoplasts were chemically fused with wild-type mesophyll protoplasts ofNicotiana plumbaginifolia. Eighty-six hybrid colonies were selected on kanamycin-containing medium, and twenty-four of these could be induced to regenerate numerous shoots. Cytological analysis of the regenerants showed the presence of a few chromosome fragments in some lines, and even a metacentric chromosome in yet another line. Besides additional chromosome fragments some lines only possessed typicalNicotianachromosomes, and this at the diploid (2n = 2X = 20) as well as the tetraploid (2n = 2X = 40) level. Biochemical analysis showed that all regenerants had neomycin phosphotransferase activity (NPTII), which suggests that intergenomic recombination and or translocation events took place at least in those lines where no additional chromosome fragments could be detected. The presence of the NPTII gene was shown by Southern hybridization. All regenerants tested were fertile, and the segregation ratios for the kanamycin gene (for self and backcross pollinations to the recipient partner) for some of the regenerants correspond with Mendelian rules for a monogenic dominant marker. Most of the regenerants showed abnormal segregation ratios; in this case, no correlation could be made between segregation ratio and chromosome composition.Our results demonstrate the existence of intergenomic recombination and translocations evens in nuclear somatic hybrid plants obtained via “gamma”-fusion.