BOTH OLIGODENDROCYTES AND ASTROCYTES DEVELOP FROM PROGENITORS IN THE SUBVENTRICULAR ZONE OF POSTNATAL RAT FOREBRAIN
BOTH OLIGODENDROCYTES AND ASTROCYTES DEVELOP FROM PROGENITORS IN THE SUBVENTRICULAR ZONE OF POSTNATAL RAT FOREBRAIN
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DOI：
10.1016/0896-6273(93)90311-e
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发表时间：
1993-02-01
期刊：
影响因子：
16.2
通讯作者：
GOLDMAN, JE
中科院分区：
文献类型：
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作者：
LEVISON, SW;GOLDMAN, JE
出生后大鼠前脑室下区（SVZ）细胞的发育命运通过逆转录病毒介导的基因转移和神经胶质抗原的免疫标记来确定。将含有 β-半乳糖苷酶的逆转录病毒立体定向注射到 SVZ 感染的小未成熟细胞中。注射后 28 天，标记细胞已出现在同侧半球的灰质和白质中。白质含有标记的少突胶质细胞，但星形胶质细胞很少，而新皮质和纹状体含有两种神经胶质细胞类型，通常出现在紧密结合的簇中。同时注射含有碱性磷酸酶和β-半乳糖苷酶的逆转录病毒后的分析表明，每个皮质簇中的细胞都是相关的。大多数簇包含单一细胞类型，但大约 15% 包含星形胶质细胞和少突胶质细胞。这些观察强烈表明单个 SVZ 细胞可以分化成两种神经胶质类型。
The developmental fates of subventricular zone (SVZ) cells of the postnatal rat forebrain were determined by retroviral-mediated gene transfer and immunolabeling for glial antigens. A beta-galactosidase-containing retrovirus injected stereotactically into the SVZ infected small, immature cells. By 28 days post-injection labeled cells had appeared in both gray and white matter of the ipsilateral hemisphere. White matter contained labeled oligodendrocytes, but few astrocytes, while neocortex and striatum contained both glial types, often appearing in tightly knit clusters. An analysis after simultaneously injecting alkaline phosphatase- and beta-galactosidase-containing retroviruses showed that cells in each cortical cluster were related. Most clusters contained a single cell type, but approximately 15% contained both astrocytes and oligodendrocytes. These observations strongly suggest that a single SVZ cell can differentiate into both glial types.