Thermal denaturation of staphylococcal nuclease.
Thermal denaturation of staphylococcal nuclease.
复制标题
葡萄球菌核酸酶的热变性。
DOI：
10.1021/bi00343a004
复制
发表时间：
1985
期刊：
影响因子：
2.9
通讯作者：
Sturtevant,JM
中科院分区：
文献类型：
--
作者：
Calderon,RO;Stolowich,NJ;Gerlt,JA;Sturtevant,JM
Materials and MethodsAll chemicals were of analytical reagent grade. pdTp (tetralithium salt, dihydrate) was obtained from Calbiochem-Boehring Corp. as A grade. Nase was isolated from homogenates of Escherichia coli cells that had been transformed with a recombinant plasmid containing the gene for the en-zyme and inducers for production of the enzyme. This plas-mid, obtained from Dr. Robert Fox of Yale University, was constructed by insertion of a Sau3A restriction fragment containing the structural gene for nuclease A in the unique BamHl restriction site in the expression vector pASl (Ro-senberg et al., 1983). This construction results in the synthesis of a modified nuclease in which the heptapeptide Met-Asp-Pro-Thr-Val-Tyr-Ser is appended to the amino-terminal alanine of nuclease A. By analogy with staphylococcalnuclease B (Davis et al., 1977), it is assumed that these extra residues
