Assessment of Circulating Sex Steroid Levels in Prepubertal and Pubertal Boys and Girls by a Novel Ultrasensitive Gas Chromatography-Tandem Mass Spectrometry Method
Assessment of Circulating Sex Steroid Levels in Prepubertal and Pubertal Boys and Girls by a Novel Ultrasensitive Gas Chromatography-Tandem Mass Spectrometry Method
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DOI：
10.1210/jc.2009-1140
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发表时间：
2010-01-01
影响因子：
5.8
通讯作者：
Le Bizec, Bruno
中科院分区：
文献类型：
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作者：
Courant, Frederique;Aksglaede, Lise;Le Bizec, Bruno
背景：雌激素和雄激素对青春期的开始和性成熟起着关键作用。大多数目前使用的免疫测定法不够灵敏，无法准确测量没有任何青春期迹象的儿童的性类固醇低循环水平。然而，这并不排除性类固醇在青春期前儿童中具有重要的生物学作用。目的：准确测定健康儿童青春期出现任何体征前血清中性类固醇激素及其代谢产物的水平，并评估可能的性别差异。主要结果指标：总（未结合的加结合的）17 β-睾酮、17 α-睾酮、5 α-二氢睾酮、5 β-二氢睾酮、雄酮、胆烷醇酮、雌二醇、和雌酮测量的超灵敏方法的基础上，气相色谱-串联质谱在样本中81名健康的学龄儿童（42名男孩）没有任何青春期的迹象。结果：17种β-雌二醇水平在青春期前男孩中检测不到或极低（中位数< 3.7 pmol/L），而在青春期前女孩中水平显著较高（中位数9.6 pmol/L，P < 0.001）。在年龄较大的青春期前儿童（>8岁）中，女孩的雄激素（4.07对1.45 nmol/L，P < 0.05）、胆烷醇酮（5.45对1.95 nmol/L，P < 0.0001）、5 α-二氢睾酮（0.11对1.95 nmol/L，P < 0.0001）、
Context: Estrogens and androgens play key roles for pubertal onset and sexual maturation. Most currently used immunoassays are not sensitive enough to accurately measure the low circulating levels of sex steroids in children without any signs of puberty. However, this does not exclude that sex steroids have important biological roles in prepubertal children.Objectives: To accurately determine levels of sex steroid hormones and their metabolites in serum of healthy children before any physical signs of puberty and to evaluate possible sex differences.Main Outcome Measures: Total (unconjugated plus conjugated) serum levels of 17 beta-testosterone, 17 alpha-testosterone, 5 alpha-dihydrotestosterone, 5 beta-dihydrotestosterone, androsterone, etiocholanolone, estradiol, and estrone measured by an ultrasensitive method based on gas chromatography-tandem mass spectrometry in samples from 81 healthy schoolchildren (42 boys) without any signs of puberty. For comparison, 48 pubertal children were studied.Results: 17 beta-Estradiol levels in prepubertal boys were undetectable or extremely low (median < 3.7 pmol/liter), whereas levels in prepubertal girls were significantly higher (median 9.6 pmol/liter, P < 0.001). Among the older prepubertal children (>8 yr), girls had significantly higher androsterone (4.07 vs. 1.45 nmol/liter, P < 0.05), etiocholanolone (5.45 vs. 1.95 nmol/liter, P < 0.0001), 5 alpha-dihydrotestosterone (0.11 vs.