STRESS DEVELOPED BY SHEETS OF CHICK FIBROBLASTS IN VITRO
STRESS DEVELOPED BY SHEETS OF CHICK FIBROBLASTS IN VITRO
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DOI：
10.1016/0014-4827(69)90299-7
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发表时间：
1969-01-01
影响因子：
3.7
通讯作者：
TAYLOR, JF
中科院分区：
文献类型：
--
作者：
JAMES, DW;TAYLOR, JF
1.1.当鸡额骨的外植体在玻璃上培养时，成纤维细胞从每个外植体的所有侧面生长出来，并在它们之间的区域形成汇合的细胞片。如果一个外植体的非面对方面的细胞分离，那么外植体通过连接它们的成纤维细胞片彼此吸引。由此产生的张力已经使用校准的微弹簧天平直接测量，并且发现在培养物之间的细胞片的横截面积约为3.4 × 104达因/cm 2。将培养物加热到40 °C以上会导致细胞片层内的张力降低（至1.7 × 104达因/cm 2），这可能表明伸展的成纤维细胞的收缩依赖于不止一种机制。
1.1. When confronted explants of chick frontal bone are cultured on glass, fibroblasts grow out from all sides of each explant, and form a confluent cell sheet in the region between them. If the cells on the non-confronted aspects of one explant are detached, then the explants are drawn towards each other by the fibroblast sheet connecting them.2.2. The tension thus developed has been measured directly using a calibrated micro-spring balance, and found to be about 3.4 × 104dynes/cm2of cross-sectional area of the cell sheet between the cultures.3.3. Heating the cultures above 40 °C caused a decrease in tension within the cell sheets (to 1.7 × 104dynes/cm2) which may indicate that contraction of an extended fibroblast depends upon more than a single mechanism.