Antimutagenicity of hydrolyzable tannins from Terminalia chebula in Salmonella typhimurium
Antimutagenicity of hydrolyzable tannins from Terminalia chebula in Salmonella typhimurium
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DOI：
10.1016/s1383-5718(98)00130-2
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发表时间：
1998-11-09
影响因子：
1.9
通讯作者：
Kaur, S
中科院分区：
文献类型：
--
作者：
Kaur, S;Grover, IS;Kaur, S
以95%乙醇和乙酸乙酯为萃取剂，成功地从慈母干果果肉中提取单宁。对TC-E进行硅胶层析，得到TC-EI、TC-EII、TC-EIII和TC-EIV四个组分。薄层色谱（TLC）和核磁共振（c -13）分析表明，TC-EI为没食子酸（GA）衍生物，其余部分为单宁。研究了TC-E及其组分对鼠伤寒沙门氏菌TA98和TA100菌株中2种直接作用诱变剂4-硝基-邻苯二胺（NPD）和4-硝基喹啉-n -氧化物（4NQNO）和s9依赖性诱变剂2-氨基芴（2AF）的抗诱变作用。结果表明，该提取物（TC-E）及其组分对s9依赖性诱变原2AF具有显著的抑制作用。在TA98中，单分子TC-EI （GA衍生物）与其他低聚组分相比效果最差，其IbD（50）值依次为TC-EIV （8.9 μ g）、TC-EIII （17.8 μ g）、TC-EII （45 μ g）、TC-EI (320 μ g)；TC-EIV对his（+）复合体的抑制效果是TC-EI的40倍。在TA100中也发现了类似的效果，其中TC-EI的效果最差，而TC-EII的效果最大。将GA（一种单体）的抗诱变性与单宁酸（一种寡聚物TA）的抗诱变性进行比较，发现了类似的结果。然而，研究发现，chebula单宁对NPD部分有效，但对4NQNO完全无效。(C) 1998 Elsevier Science B.V.版权所有
A tannin fraction (TC-E) from the dried fruit pulp of Terminalia chebula was obtained by successfully extracting with 95% ethyl alcohol and ethyl acetate. TC-E was subjected to silica gel chromatography which yielded four fractions, viz., TC-EI, TC-EII, TC-EIII and TC-EIV. Thin layer chromatography (TLC) and C-13-NMR revealed that TC-EI was gallic acid (GA) derivative while the other fractions were tannin in nature. TC-E and its fractions were evaluated for their antimutagenic potential against two direct-acting mutagens, 4-nitro-o-phenylenediamine (NPD) and 4-nitroquinoline-N-oxide (4NQNO), and S9-dependent mutagen, 2-aminofluorene (2AF) in TA98 and TA100 strains of Salmonella typhimurium. The study revealed that the extract (TC-E) and its fractions were highly significant against S9-dependent mutagen, 2AF. The effect was found to be more or less corresponding with the nature of the fractions, as the monomeric TC-EI (a GA derivative) was least effective as compared to other fractions which were oligomeric, and the order of their effectiveness as per their IbD(50) value being TC-EIV (8.9 mu g) > TC-EIII (17.8 mu g) > TC-EII (45 mu g)> TC-EI (320 mu g) in TA98; TC-EIV being 40 times more effective than TC-EI in inhibiting his(+) revertants. A similar effect was noticed in TA100 too, where TC-EI was the least effective and TC-EII had the maximum effect. A similar result was noticed when the antimutagenicity of GA (a monomeric) was compared with tannic acid (TA, an oligomeric). However, chebula tannins were found to be partly effective against NPD but not at all effective against 4NQNO. (C) 1998 Elsevier Science B.V. All rights reserved.