Ochratoxin A in wine and grape juice sold in Canada
Ochratoxin A in wine and grape juice sold in Canada
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加拿大销售的葡萄酒和葡萄汁中含有赭曲霉毒素 A
DOI：
10.1080/02652030400000653
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
P. Scott
中科院分区：
文献类型：
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作者：
W. Ng;M. Mankotia;P. Pantazopoulos;R. Neil;P. Scott
在加拿大收集了3年多的251个葡萄酒和葡萄汁样品中测定了赭曲霉毒素A（OTA）。总共有25/84份红葡萄酒样品、22/96份白色葡萄酒样品、3/46份红葡萄汁和1/25份白色葡萄汁含有高于定量限（LOQ）的OTA水平。与进口产品相比，加拿大葡萄酒的OTA发生率较低（葡萄酒的检出限为19%比48%），OTA污染水平较低（葡萄酒的上限平均值为17.5比163 pg ml−1）。来自美国的葡萄酒中没有可量化的赭曲霉毒素A。在加拿大和美国的葡萄汁样品中发现OTA，比LOD高出12.9%，上限平均值为13.3 pg ml−1。通过免疫亲和柱从葡萄酒或葡萄汁样品中提取。用水将样品基质从柱上洗掉。OTA从柱用甲醇洗脱，并通过液相色谱法使用荧光检测器定量测定。通过与三氟化硼-甲醇的酯化反应证实了OTA的存在。OTA的定量限估计在白色葡萄酒（S/N 10：1）中为20 pg ml-1，在红葡萄酒、白色葡萄汁和红葡萄汁（S/N 20：1）中为40 pg ml-1。估计白色葡萄酒的LOD为4 pg ml−1，红葡萄酒以及白色和红葡萄汁（S/N 3：1）的LOD为8 pg ml−1。
Ochratoxin A (OTA) was determined in 251 samples of wines and grape juice collected over 3 years in Canada. In total, 25/84 samples of red wine, 22/96 samples of white wine, 3/46 red grape juices and 1/25 white grape juices contained OTA levels above the limit of quantitation (LOQ). Canadian wines, when compared with imported products, showed both a lower OTA occurrence, noted as positive (19 versus 48% above the limit of detection (LOD) for wines), and a lower level of OTA contamination (upper bound mean of 17.5 versus 163 pg ml−1 for wines). Wines from the USA contained no quantifiable levels of ochratoxin A. OTA was found in Canadian and US grape juice samples, with 12.9% above the LOD and an upper bound mean of 13.3 pg ml−1. It was extracted from a wine or grape juice sample by passing it through an immunoaffinity column. The sample matrix was washed off the column with water. OTA was eluted from the column with methanol and quantitatively determined by liquid chromatography using a fluorescence detector. The presence of OTA was confirmed by esterification with boron trifluoride-methanol. The LOQ of OTA was estimated as 20 pg ml−1 in white wine (S/N 10:1) and 40 pg ml−1 in red wine, white grape juice and red grape juice (S/N 20:1). The LOD was estimated as 4 pg ml−1 for white wine and 8 pg ml−1 for red wine and white and red grape juices (S/N 3:1).