Method for morphophysiological study of specific pial microvessels.
Method for morphophysiological study of specific pial microvessels.
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特定软脑膜微血管的形态生理学研究方法。
DOI：
10.1152/ajpheart.1980.238.2.h172
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
H. Kontos
中科院分区：
文献类型：
--
作者：
W. Dietrich;E. P. Wei;J. Povlishock;H. Kontos
描述了一种方法，由此可以在生理学和形态学上研究猫软脑膜脉管系统的特定节段。这是通过插入一个颅骨窗口，通过该窗口可以在一个简单的草图中可视化、图示、编号特定的软脑膜血管，并在对照和实验条件下进行生理学评价来实现的。在这些程序之后，开始醛的灌注，并将软脑膜和相关的脉管系统从皮质套剥离。如此获得的软脑膜薄膜允许软脑膜脉管系统的直接可视化，其未改变的构型可以与其个体生理状态已知的适当的先前勾画的血管相匹配。反过来，这些血管被分成交替的节段，并准备扫描或透射电子显微镜。这种方法的优点是例证的事实，它提供了相同的微血管段的生理状态和形态特征。虽然设计用于软脑膜脉管系统，但该方案也可证明用于其他浅表微血管床的研究。
A method is described whereby specific segments of the pial vasculature of cats can be both physiologically and morphologically studied. This is accomplished by the insertion of a cranial window through which specific pial vessels can be visualized, illustrated, numbered in a simple sketch, and physiologically evaluated under both control and experimental conditions. Following these procedures, perfusion of aldehydes is initiated and the pia and associated vasculature are stripped from the cortical mantle. The thin velum of pia so obtained permits the direct visualization of the pial vasculature whose unchanged configuration can be matched to the appropriate previously sketched vessels whose individual physiological status is known. In turn these vessels are divided into alternate segments and are prepared for either scanning or transmission electron microscopy. The advantages of this methodology is exemplified by the fact that it provides the physiological status and morphological characteristics of the same microvascular segment. Although designed for the pial vasculature, this protocol may also prove of use in the study of other superficially coursing microvascular beds.