Waterlogging-induced increase in fermentation and related gene expression in the root of cucumber (Cucumis sativus L.)
Waterlogging-induced increase in fermentation and related gene expression in the root of cucumber (Cucumis sativus L.)
复制标题
涝渍诱导的黄瓜根部发酵和相关基因表达的增加（Cucumis sativus L.）
DOI：
10.1016/j.scienta.2014.10.001
复制
发表时间：
2014-11-24
影响因子：
4.3
通讯作者：
Chen Xuehao
中科院分区：
文献类型：
--
作者：
Xu Xuewen;Wang Huihui;Chen Xuehao
淹水通常导致植物细胞在缺氧胁迫下将其代谢转向厌氧发酵途径。以黄瓜耐涝品系早二N为材料，研究了下胚轴基部和第一片真叶基部两种不同程度的淹水处理对诱导黄瓜根系发酵的作用。根的活动，死亡细胞的数量，编码丙酮酸脱羧酶（PDC），乙醇脱氢酶（ADH）和乳酸脱氢酶（LDH）的基因的转录本，和PDC，ADH和LDH酶的活性，乙醛，乙醇和乳酸的浓度进行了定量，在48小时的内涝，48小时的后续恢复。结果表明：淹水过程中，PDC、ADH、LDH的转录本、活性以及乙醛、乙醇、乳酸的浓度均上调或升高，并随恢复时间的延长而降低。结果表明，淹水至第一真叶基部比淹水至下胚轴基部对根系活力和死亡细胞数的影响更大，这主要是由酒精发酵引起的。(C)2014爱思唯尔有限公司版权所有。
Waterlogging usually leads plant cells to shift their metabolism towards anaerobic fermentation pathways due to the hypoxia stress. The roles of two different levels of waterlogging treatments (water level was kept on the base of hypocotyls and on the base of the first true leaf) in induction of fermentation in waterlogged roots of cucumber line 'Zaoer-N' (waterlogging tolerant line) were examined. Root activities, numbers of dead cells, transcripts of genes encoding pyruvate decarboxylase (PDC), alcohol dehydrogenase (ADH) and lactate dehydrogenase (LDH), and activities of the PDC, ADH, and LDH enzymes, concentrations of acetaldehyde, alcohol and lactate were quantified during 48 h of waterlogging, and 48 h of subsequent recovery. The results showed that PDC, ADH, and LDH transcripts, activities of PDC, ADH and LDH, and concentrations of acetaldehyde, alcohol and lactate were upregulated or increased during waterlogging, and decreased with recovery time prolonged. The results revealed that waterlogging up to the base of first true leaf responded more severe than waterlogging up to the base of hypocotyls in root activity and numbers of dead cells, which were mainly caused by alcohol fermentation. (C) 2014 Elsevier B.V. All rights reserved.